Quantitative Analysis Of T-2 Toxin Based On Rapid Nanoresponse Method

With technological development, social progress and worldwide population growth, human gradually expand the scope of the natural development. The ensuing damage to the environment is also growing. Some ignored by us or nonexistent problems has became increasingly severe, people payed more attention to food safety and environmental pollution problems. Nowadays, food choice is not limited to fruits and vegetables, cereal grains or meat. Some usually disregarded things are on the table, and ingredients also have more different handling methods. While People enjoying various delicious, the potential risk of biotoxins is also increasing. Although there are many identified biotoxins been conducted manually detoxification, these methods may cause the introduction of new or enriched trace toxins. Fusarium and their products are extremely invasive for food crops such as wheat, barley, oats, corn, etc. T-2 toxin was widespread released in nature by Fusarium flora. Through the enrichment of organisms and the food chain, may contaminate the plant and animal food sources, and result to livestock and human beings’ health damage. However, limited to their own level or not attached enough importance, many countries don’t have specific limit standard for T-2 toxin, some even have no relevant standard. We can only find feed limits in current standards in China, and there is no explicitly stipulation on food. Existing detection methods at home and abroad for T-2 toxin mainly are thin layer chromatography, high performance liquid chromatography, enzyme linked immunosorbent assay, chromatography-mass spectrometry etc. But these methods also have their own obvious limitations. Some have too cumbersome sample processing steps and tedious operation, some need complex and expensive equipment and professional operator, some may get false-positive test results and the accuracy of the results are not satisfactory. Therefore, we take T-2 toxin as target to find a new fast, simple and efficient method for trace detection, to meet testing requirements.With their outstanding characteristics, such as quantum size effect, small size effect, surface effect and superparamagnetism, magnetic nanoparticles have been widely uesd in many fields, especially in magnetic hyperthermia, bio-separation, environmental/food microbiology detection and targeted drug delivery. Fluorescent III quantum dots also have some superior fluorescence properties, including high quantum yield, adjustable emission wavelength, broad excitation spectra, narrow emission spectra, and negligible photobleaching, has been widely used as fluorescently labeled probes. And modified quantum dots have a good biocompatibility and a relatively low toxicity to organisms.In this paper, we take T-2 toxin as a target which is no clear national standard by now, based on a specific immune response between antigen and antibody, using our own synthesized Fe3O4 nano-magnetic microspheres and Cd Se quantum dots as fluorescent detection probe, to construct a trace, fast and efficient method for the detection of biological toxins. First, through activation by EDC and NHS, get the antigen conjugated to surface of quantum dot, forming QD-Ag fluorescent detection probe, meanwhile, conjugated the corresponding antibody coated magnetic beads, formation PS-Ab magnetic separation probe, and characterize the particle size and fluorescence intensity of coupling results. Then, according to the principle of combining immunization, fluorescence detection probe and the free toxin in the analyte competitive binding antibody binding sites on the surface of magnetic separation probe. Because of the competitive binding, the concentration of the free toxin in the analyte inversely proportional to the quantum dot fluorescence intensity. By comparison with a standard curve, we achieved a quantitative analysis of T-2 toxin.Compared with the HPLC-MS and ELISA, our method is much better, which means that it is more sensitive and accurate at the level of trace detection. The detection limit is 0.01 ng/m L, the limit of quantification is 0.1 ng/m L.In this work, we developed a fast and efficient trace detection method of T-2 toxin, which has broad application prospects. And by changing the connected ligands and receptors of two probes, this method can be applied to the detection of different biological toxins, environmental pollutants or disease markers. With growing economic strength, and improving living standards, the establishment of a simple and effective detection method is not only the requirements of scientific development, also an inevitable requirement of social progress.