| For manned spaceflight,the long-term healthy life of the astronauts in the space station is the guarantee for the successful completion of the space station.The damage of the astronaut to the astronaut and the damage to the equipment can not be ignored because the space station is in a sealed condition for a long time.Reported the biological hazards alarm related key technology for space research status and problems,designed a set of array type single channel fluorescence PCR detection system for space application,including the study on the design theory of the reagent electromagnetic drive,single channel type electromagnetic driving device structures,temperature analysis system theory to set up the temperature control device,PCR amplification,in situ forming production of optical micro lens and improvement of fluorescence detection,fluorescence detection results of silver nanoparticles based on improved.Following are the main research work and achievements in this paper:1.Electromagnetic driving mechanism of array single-channel PCR fluorescence detection system is studied.An electromagnetic drive device for two types of PCR microchannel amplification systems with external electromagnetic drive and built-in electromagnetic drive has been designed.2.The electromagnetic driving mechanism of array single channel PCR fluorescence detection system was studied.The electromagnetic drive devices of external electromagnetic drive and built-in electromagnetic drive for PCR microchannel amplification system are designed.these two types of drive devices use the magnetic field generated by the electrifying coil to generate strong magnetic field force on the slider containing the magnet block.the magnetic field generated by the electrifying coil is studied,and the stress of the reagent module inside and outside the coil is calculated,which lays a theoretical foundation for the subsequent construction of real-time fluorescent PCR detection system for PCR amplification work and microstructure under the condition of space weightlessness.3.The temperature field distribution of array single channel PCR fluorescence detection system was designed,analyzed and optimized,the heating rate of the heating module of the system is studied,the steady-state temperature distribution under different gas convection conditions is studied,according to the characteristics of electromagnetic propulsion,the cooling situation of the reagent module at the tail of the pipeline was analyzed,these work provide that basis for the construction of the electromagnetic drive device.An in-situ dynamic solidification microlens is designed to improve the detection results of fluorescence detection.The mechanical mechanism of forming is studied,the light gathering properties of the ellipsoid,paraboloid and hyperboloid are compared.In addition,the method of improving the fluorescence detection results by using the surface enhanced fluorescence effect of silver nanoparticles was studied.4.An array of single-channel fluorescent PCR detection system was established based on the theory of temperature control simulation and electromagnetic module.The temperature of three temperature zones is controlled by PID algorithm,and optimized by using the critical scaling method.The principle of built-in electromagnetic drive is studied in detail,the driving process is decomposed,the relationship between that change of magnetic induction intensity and position is calculated,the relationship between magnetic induction intensity gradient and position is discussed,the movement track and reset of reagent module driven by magnetic force is discussed,the relationship between current and drive time is consistent with the change rule of simulation calculation.The temperature rise of the whole system is simulated according to the power density of the heating source,reagent module can reach the set temperature about 17 s.The temperature setting should be raised when setting the temperature so that the reagent module reaches the predetermined temperature,wherein that temperature range of 94 ? should be set to 109?,the temperature range of 72? is set to 84?,the temperature range of 56? is set to 64?As that reagent module flow cool from the 94 ? zone to the outside of the 56 ? zone,can be reduced to 56 ? in 9s when the convection coefficient is 20 w /(m2 k).In this paper,the change of line shape in the process of in-situ dynamic curing forming microlens droplet spreading is studied,the smaller that aspect ratio of the microlens,the stronger the asphericity.The aspheric properties of three kinds of aspheric surfaces-ellipsoidal surface,parabolic surface and hyperboloid surface are compared,three kinds of surface in-situ forming optical microlenses were fabricated to measure the condensing performance.For this device,the parabolic microlens has the best condensing performance,which is consistent with the calculation results.The spectral amplification of fluorescein SYBR GREEN I in the detection of biological PCR reagents is studied and silver nanoparticles is fabricated to improve the fluorescence signal,the experimental results show that the surface fluorescence signal is doubled after adding silver nanoparticles.An array single-channel PCR fluorescence detection device is made on that basic of the previous research,mainly including temperature control module,electromagnetic drive module,etc.The electronic control module is introduced,the temperature control process is optimized by a PID algorithm,the precision of 94? temperature range is±0.1?,72? temperature range is ±0.8?,56? is ±0.3?.A digital constant current source is manufactured to supply power to the drive coil,the power supply is tested,the circuit consumes approximately 3.226 W,the efficiency of the DC-DC converter is about 94.75 %. |
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