The Methods Of Microalgae Detection And Classification System For Ship Ballast Water Based On Microfluidic Chip

Ocean-going vessels as the main tool for international trade and maritime transport, which carry ballast water has become the main way to spread exotic marine organisms. Microalgae is the major detection target of ballast water, so the implementation of ballast water microalgae site rapid automatic detection and identification of microalgae species is particularly important.This paper is proposed a new method of portable ship ballast water in situ rapid detection and classification in a microfluidic chip. The main research content is as follows:We developed a novel microfluidic biosensor system that can quickly and accurately detect the viability of single microalgae cells based on chlorophyll fluorescence. To demonstrate the utility of this system, six different living and dead algae samples were tested. The developed biosensor can distinguish clearly between the living microalgae cells and the dead microalgae cells but also obtains quantitative evaluation about the activity of microalgae cells. The smallest microalgae cells that can be detected by using this biosensor are 3 μm ones. Combined with the Resistance Pulse Sensor(RPS) principle detection system used to detect microalgae, and roughly analyze of the size and count of microalgae.Based on the previous content, we presented a new system for identification of microalgae cells on a disposable microfluidic chip using simultaneous detection of 3 dimensional signals including chlorophyll fluorescence and side scattered light and Resistance Pulse Sensor (RPS). In this new method, the relations between these three signals and characteristics of microalgae cells are investigated respectively. The performance of the new system is analyzed by using three kinds of microalgae cells mixed samples compared with Flow cytometry.The research in this thesis provides a new method of microalgae survival status detection and classification for ballast water and the developed microfluidic biosensor has great potential for in situ ballast water analysis.