Screening And Preliminary Application Of Sensitive Chinese Medicines For Main Pathogenic Bacteria Of Dairy Cows With Recessive Mastitis In Jilin Province

Bovine mastitis characterized with mucosal flushing,swelling,hyperpyrexia,touch sensitivity as well as impaired mammary function,is an inflammatory disease of the udder tissue due to physical trauma or microorganisms infections.Then,subclinical mastitis tend to be more severe and easy to be ignored due to the lack of obvious clinical manifestations,which is one of most significant disease with effect on dairy cow breeding.2014,2015 and 2016 dairy cows were respectively 245,262 and 250 thousand in Jilin province.Due to subclinical mastitis incidence every year,an early,rapid and accurate diagnosis and specific treatment of mastitis would be economically valuable in Jilin province as important breeding base of dairy cow in China,For rapid and specific diagnosis and treatment of cow mastitis,this study firstly and finely investigates the incidence of mastitis in Jilin province,and analyze the relationship of morbidity with parity,seasonality,lactation stage and so on.Secondly,through bacteria isolation,cultivation,biochemical analysis and animal regression research,we identified the main pathogenic bacteria causing mastitis in Jilin province.Thirdly,with the in vitro growth inhibition,MIC and MBC test as well as analysis of the inflammatory factors HP and SAA in subclinical mastitis cow,the single Chinese herbal medicine or compound with precise therapeutic effect were picked.Finally,the therapeutic effect of Chinese medicine screened was verified through clinical efficacy test,somatic cell count and milk yield of subclinical mastitis cows.Results: The epidemiological survey shows the morbidity of cow subclinical mastitis is affected by multiple factors,such as parity,season,lactation stage,breeding and immune state.The high incidence rate of subclinical mastitis happens when it is above five parity,from July to September,and at the peak of lactation.The Escherichia coli,Staphylococcus aureus,and Streptococcus agalactiae are the main pathogenic bacteria in Jilin province.Especially,E.coli infection is most and accounted for 37.45%,followed by S.aureus infection with 36.70% incidence and S.agalactiae infection with 24.34% incidence.The screening result for main pathogenic bacteria showed Dandelion,Houttuynia cordata,motherwort,safflower and Lonicera japonica Thunb can effectively inhibit and kill pathogenic E.coli,S.aureus and S.agalactiae,proving the drugs above as alternative drugs.The compound chinese medicine composed of Herba Leonuri,Honeysuckle,Forsythia suspensa,Dandelion,Luffa sponge,Tongcao and Fritillaria thunbergii shows better effect on treating subclinical mastitis in Jilin province,which obviously improve milk yield,decrease somatic count and increase cure rate.The results show this Chinese medicine compound could be applied to treat cow subclinical mastitis.This study has proven to be effective in clinical research.Once applying to breeding widely,this Chinese medicine may not only decrease the incidence rate,improve distinct economy,but also provide high-quality milk and beef for people which is helpful to improving our green food safety environment.