| The invasive plant Cenchrus spinifex can grow in arid and semi-arid areas worldwide,possibly due to its special drought tolerance properties.In this study,isobaric tags for relative and absolute quantitation(iTRAQ)was employed to investigate the molecular mechanisms of C.spinifex drought-tolerance,resulting that 385 differentially accumulated proteins(DAPs)were detected in drought stress roots at low and middle soil moisture(LM and MM)compared to high soil moisture(HM,hereafter,as the control).Based on the proteomic analysis of DAPs,the C.spinifex drought response mainly involves high physiological metabolic activities,including protein biosynthesis,product transport and energy utilization;the accumulation of flavonoid intermediates;and reactive oxygen species(ROS)scavenging based on the ascorbate-glutathione(ASC-GSH)cycle.The results of physiological and biochemical assays showed that the plants suffered drought stress increased contents of sucrose,fatty acid and ASC and NADH/NAD ratio,but decreased lignin and anthocyanin contents as well as actin protein levels in comparison with the control,in line with the proteomic data.These results indicate that C.spinifex has novel molecular mechanisms that control its drought-tolerance and facilitate its spread,especially in arid and semi-arid areas worldwide.In addition,the VIGS transformation platform was successfully established to reveal the molecular mechanism of drought resistance of C.spinifex.by using Alternanthera philoxeroides as a model plant.It will be great helpful to further explore the relationship between invasion mechanism and phenotypic plasticity of C.spinifex. |
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