Mechanism Of Green Tea Polyphenol Regulated Albumen Quality Of Hens During The Late Laying Period

In this study,we studied the potential regulatory mechanisms of green tea polyphenol improved albumen quality of Hy-Line brown hens during the late laying period.Experiment 1:The effects of dietary GTP levels on albumen quality in hens during the late laying period.A total of 240 70-wk-old Hy-Line Brown laying hens were randomly divided into 4 treatments groups with 6 replicates of 10 birds each.The feeding trial lasted for 12 wks after a 1-wk adaptation period.Dietary GTP addition increased laying performance and albumen quality(P<0.05).Dietary GTP inclusion level from 200 to 400 mg/kg improved the plasma protein and lipid metabolism.Hens fed 200 mg/kg GTP improved antioxidant status in plasma,liver and magnum.The organ's unfavorable changes were modulated by dietary supplementation 200 mg/kg GTP comparable to the control group.Experimant 2:Mechanisms of green tea polyphenol improved albumen quality of Hy-Line brown hens during the late laying periodA total of 120 70-wk-old Hy-Line Brown laying hens were divided into two treatments in the absence and presence of 200 mg/kg GTP.The protein carbonyl content was decreased and the protein sulfhydryl content was increased in the albumen and magnum by the effect of GTP.A total of 3 803 proteins were identified and 264 differential proteins were observed with and without GTP.Five high abundance proteins(ovalbumin,ovotransferrin,ovomucoid,lysozyme and ?-ovomucin)in albumen were upregulated by GTP diet.Differential proteins involved in regulating antioxidant system.In summary,dietary supplementation 200 mg/kg GTP are suitable for enhanced performance,albumen quality,antioxidant defense systems and health status of organ in an aged layers diet.And the mechanisms of GTP improved the secretion of high abundance proteins in albumen through regulating cellular antioxidation capacity of magnum in aging hayers.