| Objective: To establish the microRNAs expression profile based on the results of microRNA microarray from type 2 diabetes and normal glucose tolerance group of Uygurs and Kazaks populations in Xinjiang;and the select the microRNAs with differential expression were selected as the targets,which are verified by real-time quantitative PCR,and the potential function of the target microRNAs were investigated in human adipose tissue and 3T3-L1 preadipocytes.Finnally,their basic mechanism in type 2 diabetes were disused.Methods: Based on the inclusion and exclusion criteria,microRNA microarray were adopted to select the target microRNAs with differential expression in type 2 diabetes and normal glucose tolerance group of Uygurs and Kazaks.The result from array were analyzed with SAM?volcano plots?signal pathway and target gene prediction.Then microRNA10 b,29a,143 were selected and verified by real time quantitative PCR,in human adipose tissue from different groups of Uygurs and kazaks population.Anthropometric index(blood pressure,body weight,BMI)and clinic index(FBG,TG,CHO,HDL-C,LDL-C,FINS,fasting C-P)were compared between Uygurs and kazaks population,and their correlation with microRNA10 b,29a,143 were transfected into 3T3-L1 preadipocytes,cell and supernatant were collected at different time points for Western-blot,glucose content,measurement with glucose oxidase method,and ELISA was used to check the level of IL-10,IL-6,adiponectin and leptin.Results: The microRNA expression profile of Uygurs and Kazaks was estabolished with the results from microRNA array;The confirmed results of the expression of microRNA10 b,29a,143 from real-time was matched with the results from microarray;and the expression of microRNA10 b,29a,143 has no significant difference in adipose tissue from different groups of Uygurs;Compared with NGT group in Uygur,the body weight,BMI,FBG,TC,FINS and HOMA-IR in T2 DM group were increased,and HDL-C reduced.In Kazaks,the level of FBG,LDL-C and HOMA-IR were increased in T2 DM group compared with NGT group,the level of HOMA-IS and C-P reduced;In Uygurs,microRNA143 is positively correlated with HDL-C and LDL-C;microRNA10b is positively correlated with HDL-C;but microRNA29 a is positively correlated with HDL-C,systolic pressure and LDL-C;The content of triglyceride was increased with the tansfection of microRNA29 a and microRNA143 mimics,but decreased with microRNA10 b mimics;The content of glucose was decreased with microRNA29 a and microRNA143 mimics,but increased with microRNA10 b mimics;The levels of IL-10 and leptin were decreased with transfection of microRNA143 inhibitor,but adiponectin increased;So with microRNA29 a inhibitor;The levels of IL-10 and adiponectin were increased while leptin decreased;After transfection of microRNA10 b mimics the levels of IL-10 and adiponectin were increased;then the level of leptin was decreased with microRNA10 b inhibitor;The expression of NCOR2 protein increased after transfection of microRNA10bmimics;and the expression of NCOR2 protein was increased after the transfection of microRNA10 b mimics,and decreased with its inhibitor;PPAR alpha protein expression was increased with microRNA10 b mimics and inhibitor;the expression of NCOR2 protein wsa increased with microRNA29 a mimics and inhibitor;MAPK7 protein expression was decreased with microRNA143 mimics and inhibitor.Conclusion: Established the microRNAs expression profile through the analysis of the results of microRNA microarray in Uygurs and Kazaks in Xinjiang;The expression of microRNA10 b,29a,143 is correlated with lipids in Uygurs;microRNA10b can inhibit 3T3-L1 adipocytes adipogenisis?improve insulin resistance and inhibit inflammation;microRNA29a and143 can promote 3T3-L1 adipocytes adipogenisis?increase insulin resistance;The three microRNA may be involved in the pathogenesis of type 2 diabetes. |
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