The Research On Expression And Function Of TRIM21 In Ovarian Cancer

Background:Ovarian cancer ranks among the top three malignancies in women. Tumor metastasis and drug resistance is the main reason for poor prognosis of ovarian cancer. From the collection of clinical data analysis, in patients undergoing surgery and other treatment, a high proportion of patients receiving treatment after the recurrence. Although a large number of studies have explored the mechanism of ovarian cancer progression and identified many oncogenes and tumor suppressor genes, the detailed mechanism of ovarian cancer development is still not perfect. Therefore, the discovery of new therapeutic targets or markers, may provide strong support for future clinical treatment. Tripartite motif (TRIM) family proteins are a class of conserved proteins. Some studies have shown that members of the TRIM family participate in many physiological processes such as cell proliferation, differentiation, innate immunity, apoptosis regulation, and cell motility. In recent years, studies have suggested that the TRIM protein family is closely related to tumorigenesis. TRIM21 has long been recognized as a regulator of immune responses such as antiviral infection, regulation of inflammatory responses, regulation of cytokines and secretion of chemokines. We found that TRIM21 in hepatocellular carcinoma in low expression, and can regulate the proliferation of liver cancer cells, metastasis and apoptosis, revealing the function of the molecule in the tumor. However, the expression of TRIM21 in ovarian cancer has not been reported.Method:TRIM21 expression in ovarian cancer was confirmed by immunohisto-chemistry and quantitative real-time PCR. We also analyzed the association between TRIM21 and ovarian cancer by using a network database. We also examined the expression of TRIM21 (CCK8), clonogenic ability (platelet cloning assay), metastasis and invasion (transwell assay), apoptosis level of TRIM21 in the knockdown or overexpression of ovarian cancer cell lines, respectively. (Flow cytometry).Results:TRIM21 expression was significantly lower in ovarian cancer tissues than in high-expression patients; the low expression of TRIM21 could promote the malignant phenotype of ovarian cancer cell lines (proliferation and metastasis, Invasion and anti-apoptosis), high expression can inhibit the malignant phenotype of ovarian cancer (proliferation, metastasis, invasion, anti-apoptosis). We also explored the possible physiological effects of TRIM21 in tumor tissue, including angiogenesis and drug sensitivity through bioinformatics analysis.Conclusion:This study revealed a new tumor suppressor gene, TRIM, which provides a new marker and candidate therapeutic target for diagnosis and treatment of ovarian cancer.