Sonic Hegdehog Stimulates The Migration Of MCF-7 Breast Cancer Cells Through Rac1

Breast cancer is the most common female cancer worldwide.In order to improve the therapeutic effect of breast cancer,it is urgent to have a more comprehensive understanding of its biological basis.Recently it has been demonstrated that Hegdehog signaling pathway is required for a variety of tumors for their growth and proliferation.Studys about breast cancer were proceeded with mouse mammary cancer models for Hh-dependent mechanisms of mammary gland tumor formation and development.Inhibition of Hh can reduce the survival ability of tumor cells and reduce the abnormal stimulation of basal cells.Therefore,the key molecules of the Hh signaling cascade can be used as drug targets,and combined with other drugs,to make a contribute to clinic breast cancer treatmentIn this study,we showed that:1.Shh stimulates the migration of MCF-7 breast cancer cells;2.Smo and Glil are involved in the regulation of Shh on the migration of MCF-7 breast cancer cells.Activating Smo and Glil can induce cell migration,while specific inhibitors block it;3.The effect of Shh signaling pathway on MCF-7 breast cancer is independent on Wnt5a,Dvl2 and Rab35,but directly on Rac1.To sum up,we found that in MCF-7 breast cancer cells,both the Shh and Wnt5a signaling pathways regulate the activation of Racl and thus affect the cell migration,independently.Migration of cancer cells is a complicated process by many signaling pathways,exploring the molecular mechanism of cancer cell migration is crucial for accuratly selecting therapeutic targets during clinical treatments,and appropriating combination of a variety of signaling pathway inhibitor,for the goal of improving the treatment effect,reducing the incidence of drug resistance.