The Studies On The Function Of Rac1 In The Invasion And Migration Of Breast Cancer Cell

Cancer is one of the main factors that threaten human health.Among these,breast cancer has become one of the most common cancers in women.It's difficult to cure and prognosis which mainly leads from the invasion and metastasis of tumor cells.So it's critical to further understand the mechanism of tumor metastasis for the treatment of malignant tumors.Tumor invasion and metastasis is a multi-step,complicated process,in which the migration of tumor cells plays a key role.The migration of tumor cells in the substrate includes five steps: protrusion of the leading edge;cell-matrix interaction and formation of focal contacts;recruitment of surface proteases to ECM contacts and focalized proteolysis;cell contraction by actomyosin;detachment of the trailing edge.The dynamic changes of cell actin cytoskeleton and attached structures are the basis of tumor cell migration.The dynamic changes of cell actin cytoskeleton and the formation of cellular pseudopodia are key factors in regulating tumor cells migration and movement.Some proteins which are related to cell actin cytoskeleton regulation such as Rho GTPase family play an important role in cell migration.Studies have shown that the Rac1 is primarily related to the formation of lamellipodium and cell membrane bumps in the tumor cell migration.In this paper,we study the function and mechanism of Rac1 in the invasion and migration of breast cancer cell.Firstly,by the experiments of wound healing we show that malignant MDA-MB-231 cells have more obvious lamellipodium,cytoskeleton and higher ability to migrate than the MCF-7 cells.Further,it's found that there is a positive correlation between the high ability to migrate of MDA-MB-231 cells and the expression levels of Rac1 protein.By the RNA silencing technology and inhibitors inhibit experiment,We found that it can significantly inhibit the MDA-MB-231 cells' ability to migrate by reducing the expression levels of Rac1 or inhibiting its activity.We preliminary evidence that Rac1 may through the Rac1-PAK signaling pathways to regulate the migration of MDA-MB-231 cells through the use of PAK1 inhibitors.Related outcomes for the further research of breast cancer treatment provide a good basis.