Study On The Effect Of Laminin A5 In Preeclampsia

ObjectiveTo determine the expression and location of LN-a5 in the placenta of preeclampsia(PE).To determine the expression and function of LN-a5 in HTR8/SVneo and HUVEC cells.To investigate the role of si LN-a5 in PI3K/AKT signaling pathway and explore its role in the pathogenesis of PE.Methods1 The expression and location of LN-a5 in PE placenta were detected by qRT-PCR,Western blotting and immunohistochemistry.2 LN-a5 was downexpressed by siRNA LN-a5,the transfection effifiency was identified by qRT-PCR,Western blotting and immunohistochemistry.3 After trsnsfected successful,the proliferation of HTR8/SVneo and HUVEC cells was detected by CCK-8.The invasion of HTR8/SVneo and HUVEC cells was detected by matrigel transwell assay.The angiogenic ability of HUVEC cells was detected by tube formation assay.4 HTR8/SVneo and HUVEC cells were used to mimic the effects of a Hypoxia-Reoxygenation(H/R)environment on placentas to study LN-a5 expression and function of proliferation,apoptosis,invasion and angiogenic ability.5 The effect of LN-a5 in PI3K/AKT signaling pathway was detected by Western blotting.Results1 Our result shows that gene and protein levels of LN-a5 was significanty lower in PE compared with the control placentas.2 After downexpressed the LN-a5,the proliferation and invasion of HTR8/SVneo and HUVEC cells were increased,the angiogenic ability of HUVEC cells were decreased.3 After HTR8/SVneo and HUVEC cells were treated with H/R,the proliferation and invasion of HTR8/SVneo and HUVEC cells were increased,the apoptosis of HTR8/SVneo and HUVEC cells were decreased,the angiogenic ability of HUVEC cells were decreased.4 The phosphorylation levels of PI3K signaling pathway was depressed after treated with H/R in HTR8/SVneo cells.ConclusionLN-a5 involved in regulating the function of HTR8/SVneo and HUVEC cells,which suggested a possible pathological mechanism of PE.