Study On Inhibition Of Bacterial Biofilm Formation By Copper Ion Implantation Of Titanium Alloy

OBJECTIVEBacteria adhere to and reproduce on the surface of internal plant materials,forming biofilm and causing internal plant infection are the main reasons for failure of internal plant fixation in clinic.Titanium alloy,a commonly used plant material in orthopaedics,lacks self-infection resistance.In this study,the surface of titanium alloy was modified by ion implantation technology,and copper and silver were implanted on the surface of titanium alloy.The anti-bacterial biofilm properties of the modified titanium alloy were mainly discussed.METHODSThe surface of titanium alloy was modified by metal vapor vacuum arc(MEVVA)ion implantation.Field Emission Scanning Electron Microscope(SEM)was used to observe the surface morphology of titanium.Energy Dispersive Spectrometer(EDS)was used to analyze the elements on the surface of titanium.The growth of bacteria on the surface of the material was observed.The ability of the modified titanium alloy to inhibit the biofilm was tested by counting the number of live bacteria and laser confocal microscopy.Animal models of osteomyelitis associated with internal plants were used to verify the antimicrobial activity of the materials in vivo.Bone marrow stem cells(BMSCs)were used to detect the biocompatibility of titanium modified by cell adhesion,proliferation and morphology on the surface of the material in vitro;and RhoA protein level of the cells was tested to verify the possible fineness.Cellular pathways.Animal models were used to validate the osteointegration effect of the materials in vivo.RESULTSNanoparticles of copper could be implanted on the surface of titanium by MEVVA ion implantation technology;the number of implanted particles was emerged with increase of implantation time.The colonized copper and silver particles inhibited the formation of biofilm on the surface of titanium and reduced the incidence of plant infection.Biocompatibility experiments confirmed that the material had no obvious cytotoxic effect,improved the early adhesion ability of bone marrow stem cells.CONCLUSIONWith MEVVA technology,nano-copper and silver can be implanted on the surface of titanium controllably,which endows titanium with the ability of anti-biofilm and bone integration.