| Purpose: The aim of this research was to evaluate the biological safety and compatibility of the selective-laser-melted antibacterial titanium alloy Ti6Al4V-6Cu.Furthermore,mandibular osteoblast cell sheet was constructed on the alloy surface.Methods: 1.Cytotoxicity test,hemolysis test and acute general toxicity test were carried out to evaluate the biological safety of the antibacterial titanium alloy Ti6Al4V-6Cu.2.SD rat mandibular osteoblasts were cultured in vitro and seeded on the surface of Ti6Al4V-6Cu.In order to evaluate the biocompatibility of the antibacterial titanium alloy Ti6Al4V-6Cu,cell proliferation and differentiation were detected by MTT,alkaline phoephatase(ALP)activity,mineralized nodules formation and real-time RT-PCR.Cytoskeleton was observed by phalloidin and DAPI staining.3 ? SD rat mandibular osteoblast cell sheet was constructed on the surface of Ti6Al4V-6Cu by culture medium supplemented with vitamin C.Visual inspection,HE staining,were employed for morphological observation of the sheet.Cell sheet differentiation was detected by alkaline phoephatase(ALP)activity and real-time RT-PCR.Results: 1?Selective-laser-melted antibacterial titanium alloy Ti6Al4V-6Cu has no cytotoxicity,no haemolysis and no acute systemic toxic.Suggesting that the material has good biosecurity.2?Titanium alloy Ti6Al4V-6Cu presents good biocompatibility.SD rat osteoblasts seeded on the surface grow well.Ti6Al4V-6Cu is favorable for the adhesion proliferation and differentiation of mandibular osteoblasts.3?Mandibular osteoblast cell sheet was constructed on the surface of titanium alloy Ti6Al4V-6Cu in growth medium supplemented with vitamin C.The cell sheet was full of extracellular matrix and had osteogenic potential.Conclusions: Selective-laser-melted antibacterial titanium alloy Ti6Al4V-6Cu has good security and biocompatibility.Mandibular osteoblast cell sheet can be constructed successfully on Ti6Al4V-6Cu surface with vitamin C medium. |
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