Studies Of Diabtic Wound Healing And Angiogenesis Effcts Of Hydrogel Carring Deferoxamine Mesylate

Diabetic wound nonunion and delayed union is always a difficult problem which has not been overcome in medicine.So there is need for dressing that can coverage wound in early,promote angiogenesis and accelearate wound healing at the same time in clincal.As a chelating agent of iron,deferoxamine mesylate(DFO)is showing a potential ability to heal soft tissue wound,but it has few applications in wound repair,and a little is known about the process and mechanisms of healing.In the present study the Deferoxamine grafted alginate SA-DFO with sodium alginate SA as the main chain and DFO as side chain was successfully created.The basic properties and self repair behavior of the modified alginate SA-DFO were studied,and evaluated in vitro and in vivo.Give different amounts of DFO when creat SA-DFO will affect the number of branched chains.According to the different proportions of SA and DFO,SA-DFO is divided into three groups: SA-DFO1(SA:DFO = 10:3),SA-DFO2(SA:DFO = 5:2),and SA-DFO3(SA:DFO = 2:1).When meet with fluid the SA-DFO can convert to hydrogel spontaneously which has self-healing capabilities and release the side chain DFO slowly.In vitro cell culture showed that the release products of the SA-DFO hydrogel was not toxic to human umbilical vein endothelial cells(HUVEC),bone marrow derived mesenchymal stem cells(BMSC)and mouse fibroblast(L929),promoted HUVEC proliferation,HUVEC,BMSC,L929 migration,HUVEC tube formation and secretion of wound healing related growth factor and stimulated the expression of angiogenic-related genes of the fibroblasts and human umbilical vein endothelial cells.In vitro,we induced diabetic rats by intraperitoneal injection of streptozotocin(STZ),then 2*2cm full thickness skin defect was cut on the rats back to establish diabetic wound models,and treated in each group.When used to treat full-thickness skin defects in diabetic rats,the SA-DFO hydrogel showed a significantly better capacity to stimulate wound healing,collagen deposition,maturity reepithlialization and angiogenesis than the alginate and the untreated defects.In addition,the results both in vivo and vitro showed that the SA-DFO2 hydrogel was the best in the three groups of SA-DFO.These results indicate that Wound dressings composed of alginate hydrogels carrying Deferoxamine mesylate not only can protect wound from external harmful factors but also have a promising capacity to heal full-thickness skin defects and stimulate angiogenesis,and SA-DFO2 hydrogel had the optimal drug release concentration.It is believed that this result can promote the follow-up clinical research and promotion of SA-DFO hydrogel dressing,and provide valuable data for understanding the role of the hydrogel-based dressing carrying drug in healing soft tissue wounds.