| BackgroundSevere acute pancreatitis?SAP?is a serious and complex disease which can lead to many complications.Systemic inflammatory response syndrome?SIRS?often caused by SAP can lead to lung injury?ALI?,acute respiratory distress syndrome?ARDS?,severe infection and multiple organ dysfunction syndrome?MODS?.The mortality rate of SAP is very high.The clinical treatment of SAP include conservative treatment or surgical treatment.A large number of clinical studies confirmed that surgical treatment for SAP may cause surgical trauma and inflammatory reaction which can aggravate pancreatic and systemic injury,and will cause severe secondary infection,lung,liver,kidney,heart and other organs function failure.The incidence rate of severe acute pancreatitis in China increased in recent years,but there are lack of the effective treatments to improve the cure rate and survival rate.So the pathogenesis and effective treatment of severe acute pancreatitis is a difficult international clinical problems.Severe acute pancreatitis is a multifactorial and complex pathophysiological process.The present study shows that the main pathogenesis of SAP include trypsin autodigestion intestinal bacterial translocation excessive activation of inflammatory cells,calcium overload,pancreatic circulatory disorder and high blood fat,etc..Digestion enzymes in pancreas were activated and various cytokines were released sustained from a large number of inflammatory cells.Inflammatory mediators trigger a cascade of continuous inflammatory response which leads to multiple organ dysfunction.Pancreas,as the primary organ,is often the most severely damaged organ.Lung as the most common target organ of SIRS is the most easily affected organ,and lung injury is one of the most common complications of SAP.Studies in recent years show that the sustained release of inflammatory factors is one of the main factors that cause the deterioration of SAP.Therefore,the focus of the clinical treatment of SAP is to reduce the inflammatory response,to maintain the balance of pro-inflammatory and anti-inflammatory responses,blocking MODS caused by SIRS.So the inflammatory signaling pathway,expression and regulation of inflammatory factors have become a hot topic in international research.Heme-1?HO-1?is the rate limiting enzyme of heme degradation and is also known as heat shock protein-32?HSP-32?which protects cells and reduces oxidative stress in cells.HO-1 also has a series of cellular processes such as cell growth,inflammation,apoptosis,and so on.Some studies suggest that HO-1 can reduce the tissue damage caused by the inflammatory response induced by different proinflammatory factors.In some animal experiments,organs damage increased and mortality increased in sepsis model rats established by lipopolysaccharide?LPS?which were lack of HO-1 gene or treated with HO-1 inhibitors.Other studies show that HO-1 play an important role in the regulation of oxidative stress,inflammatory reaction,and play a role in organ protection.TNF-a and IL-10 are important pro-inflammatory and anti-inflammatory cytokines.IL-10 can inhibit the release of IL-2,IL-3,TNF-a and other cytokines,can also relieve inflammatory reaction and protect organ function.IL-10 has the potential anti-inflammatory function and protective effect on multiple organ injury caused by SAP.TNF-a is a cytokine released by activated macrophages and lymphocytes which can increased the expression of IL-6 and IL-8 which leading to cell damage and tissue destruction.In recent years,some studies have found that HO-1 can regulate the expression of IL-10 and mediates the anti-inflammatory effect of IL-10.In macrophages of mouse,IL-10 can induce HO-1 expression through the p38-MAPK signaling pathway.In a mouse model of septic shock induced by LPS,the anti-inflammatory action of IL-10 can be attenuated by HO-1 inhibitor ZnPP.Recent studies have found that the induction of HO-1 gene expression is related to multiple signal transduction pathways,and various inflammatory signal factors induce the expression of HO-1 gene through different inflammatory signal transduction pathways.Inflammatory signal transduction pathways inducing HO-1 gene expression can be divided into:?1?p38/MAPK inflammation signal transduction pathway;?2?PI3K/AKT inflammation signal transduction pathway?post-transcriptional regulation?;?3?JAK/STAT inflammation signal transduction pathway;?4?protein kinase pathway.Among them,mitogen activated protein kinase?MAPK?inflammation signal transduction pathway has been proved to be the key way to induce HO-1 gene expression.However,the regulation of p38 MAPK/NF-kappa B inflammatory signal transduction pathway activated by HO-1 has not been reported at home and abroad.There were no conclusion that whether the expression of HO-1 can regulate inflammatory factors TNF-alpha and IL-10,whether it can protect pancreas and lung,and whether these effects of HO-1 acted through p3 8 MAPK/NF-kappa B inflammatory signal transduction pathway in SAP.If we can block the excessive activation of systemic inflammatory response from the inflammatory signal transduction pathway,it will provide a powerful method for the clinical treatment of SAP which will have important clinical significance.OBJECTIVE:To observe the effect of heme oxygenase-1?HO-1?overexpression and inhibition on TNF-a?IL-10?HO-1 content of serum,pancreas and lung tissue,and pathological changes of pancreatic and lung tissue in rats with severe acute pancreatitis?SAP?.To observe the regulatory effect of HO-1 on p38 MAPK/NF-kappa B inflammatory signal transduction pathway in pancreas and lung tissue of SAP rats.To explore the protective effect of heme oxygenase-1 in pancreas and lung inflammatory reaction,and analysis the probable effective mechanism of HO-1 on pancreas and lung.METHODS:60 male SD rats?6 to 7 weeks old,weight of 220-260g?were randomly divided into 4 groups:control group,SAP model group,HO-1 over expression group and HO-1 inhibitor group,and 15 rats in each group.1.the control group:sham operation group,only open the abdominal cavity,and then closed,normal saline lOml/kg was injected intraperitoneally into the rats,free drinking water after operation.2.the SAP model group:the SAP model was established by retrograde pancreatic duct injection of 5%sodium taurocholate?0.lml/100g?,free drinking water after operation..3.the HO-1 inhibitor group:ZnPP?Zn-protoporphyrin,HO-1 inhibitor?20tmol/kg was injected into the rats intraperitoneally 30 minutes after the SAP model was established.4.the HO-1 over expression group:hemin?HO-1 accelerator?75?g/kg was injected into the rats intraperitoneally 30 minutes after the SAP model was established.Rats were sacrificed 24hs after the model was made.Blood samples were collected from abdominal aorta,and pancreas and lung tissue samples were collected.Pancreatic and lung tissue paraffin sections were prepared.To observe the pathological changes of pancreatic and lung tissue by HE staining and immunohistochemical staining.The contents of serum IL-10,HO-1 and TNF-a were detected by ELISA method.The contents of IL-10,HO-1 and TNF-a of pancreas and lung tissue homogenate were detected by ELISA method.Total proteins were extracted from pancreas and lung tissues.The expression levels of key proteins of p38 MAPK/NF-kappa B pathway?p38 MAPK,phosphorylated P38 MAPK,MAPKAPK2,and NF-kappa B p65?was detected by Western Blot method.RESULTS:Compared with the control group,the serum contents of HO-1,IL-10 and TNF-a in the SAP group were elevated?0.8510.14 vs 0.2810.03,71.89±8.91 vs 11.05±1.79,29.26±3.69 vs 12.5312.04,P<0.01?,the contents of HO-1,IL-10 and TNF-a in pancreas tissue were higher in the SAP group?0.52±0.08 vs 0.1810.02,52.63±6.02 vs 26.37±3.57,31.76±5.52 vs 14.78± 2.39,P<0.01?,the contents of HO-1,IL-10 and TNF-a in lung tissue were higher in the SAP group?0.46±0.06 vs 0.09±0.01,49.18±6.80 vs 19.51±2.92,29.61±3.89 vs 11.36±1.64,P<0.01?.Compared with the HO-1 over expression group,the contents of HO-1 and IL-10 in the serum were significantly decreased in the HO-1 inhibitor group?0.75±0.11 vs 1.02±0.16,6455±7.69 vs 99.83±13.33,P<0.01?,and the contents of TNF-a were significantly increased?3436 ± 3.95 vs 21.69 ± 2.95,P<0.01?.The contents of HO-1 and IL-10 in pancreas tissue were significantly decreased in the HO-1 inhibitor group?0.37±0.06 vs 0.82±0.12,48.09±6.22 vs 62.71 ±9.11,P<001?,and the contents of TNF-a were increased?34.17± 5.16 vs 25.68± 3.49,P<0.05?.The contents of HO-1 and IL-10 in lung tissue were decreased in the HO-1 inhibitor group?0.33±0.05 vs 0.79±0.11,44.72±6.67 vs 58.34±7.81,P<0.01?,and the contents of TNF-a were increased?35.06±4.92 vs 22.58±3.32,P<005?.Compared with the SAP model group,the contents of HO-1 and IL-10 in the serum were significantly increased in the HO-1 over expression group?1.02±0.16 vs 0.85±0.14,9983± 13.33 vs 71.89±891,P<0.01?,and the contents of TNF-a were significantly decreased?21.69 ± 2.95 vs 29.26± 3.69,P<0.01?.The contents of HO-1 and IL-10 in pancreas tissue were higher in the HO-1 over expression group?0.82±0.12 vs 0.52±0.08,62.71 ±9.11 vs 52.63±6.02,P<0.05?,and the contents of TNF-a were decreased?25.68±3.49 vs 31.76±5.52,P<0.05?.The contents of HO-1 and IL-10 in lung tissue were higher in the HO-1 over expression group?0.79±0.1 1 vs 0.46±0.06,58.34±7.81 vs 49.18±6.80,P<0.05?,and the contents of TNF-a were decreased?22.58±3.32 vs 29.61 ±3.89,P<0.05?.Morphology observation of pancreas:1.Gross specimen observation of pancreas:Pancreatic tissue of control group no edema,hyperemia,pancreatic necrotic,and no remarkable pathological changes;Pancreatic tissue of SAP model group was edematous,pale,and adipose tissue scattered punctate saponifying spots;Pancreatic tissue of HO-1 inhibitor group was edematous,pale,and adipose tissue scattered punctate saponifying spots;Pancreatic tissue of HO-1 over expression group was edematous,pale and no obvious saponifying spots.2.Microscopic observation:In control group,the pancreas acinar arrangement was rule,glandular morphology was normal,and there is no obvious pathologic changes.In the SAP model group,pancreatic interstitial was congestive and edematous,accompanied by inflammatory cell infiltrated;Part of the pancreas was congestive,necrotic,and alveolar arranged in disorder.In HO-1 inhibitor group,pancreatic interstitial was hyperemia and edematous obviously,inflammatory cell infiltrated,massive pancreatic were congestive and necrotic,alveolars were edematous and arranged in disorder;Pathological changes were seriously compared with the SAP model group.In HO-1 over expression group,pancreatic interstitial was edematous,small amount of inflammatory cell infiltrated;Pathological changes were lighter compared with the SAP model group.3.Pathological score of pancreatic tissue:Pathological score of pancreatic tissue in the HO-1 inhibitor group was significantly increased?13.50 ± 0.76 vs 7.00±0.49,P<0.01?compared with the the HO-1 over expression group.Pathological score of pancreatic tissue in the HO-1 over expression group was significantly reduced?7.00±0.49 vs 11.50±0.53,P<0.05?compared with the SAP model group.4.Immunohistochemistry test results of IL-10 in pancreatic tissue:The expression of IL-10 in the control group was normal and no significant pathological changes.The expression of IL-10 in model group increased and immunohistochemical staining was more obvious compared with the control group.The expression of IL-10 increased in the HO-1 inhibitor group compared with SAP model group and immunohistochemical staining was more obvious compared with SAP model group,but lighter than HO-1 overexpression group.The expression of IL-10 increased significantly in the HO-1 overexpression group and immunohistochemical staining was more obvious compared with SAP model group.5.Immunohistochemistry test results of TNF-ain pancreatic tissue:The expression of TNF-a in the control group was normal,and no significant pathological changes.The expression of TNF-a in model group increased significantly and immunohistochemical staining was more obvious compared with the control group.The expression of TNF-a increased in the HO-1 overexpression group and immunohistochemical staining was lighter compared with SAP model group.The expression of TNF-a increased significantly in the HO-1 inhibitor group compared with SAP model group and immunohistochemical staining was more obvious compared with SAP model group and HO-1 overexpression group.Pathological observation of lung tissue:1.Gross specimen observation of lung:Lung tissue of control group was no edema,hyperemia and no remarkable pathological changes.Lung tissue of SAP model group was edematous,pale.Lung tissue of HO-1 inhibitor group was edematous,pale obviously and pathologic changes was more seriously compared with SAP model group.Lung tissue of HO-1 over expression group was edematous,pale and pathologic changes was lighter compared with SAP model group.2.Microscopic observation:In control group,the structure of lung tissue was complete,clear and the cell arrangement was rule.There was no inflammatory cell infiltration and no congestion,edema,alveolar.There is no obviously pathologic changes.In the SAP model group,the structure of lung tissue was fuzzy,a large number of inflammatory cells infiltrated,a small amount of red blood cell effusion,and alveolar wall was thickening.Interstitial of lung tissue was congestive and edematous,accompanied by inflammatory cell infiltrated.In HO-1 inhibitor group,the structure of lung tissue was disordered,a large number of inflammatory cells infiltrated,a small amount of red blood cell effusion,alveolars were edematous and arranged in disorder.Pathological changes were seriously compared with the SAP model group.In HO-1 over expression group,the structure of lung tissue was complete,a large number of inflammatory cells infiltrated,a small amount of red blood cell effusion.Pathological changes were lighter compared with the SAP model group.3.Pathological score of lung tissue:Pathological score of lung tissue in the HO-1 inhibitor group was significantly increased?12.28±0.83 vs 5.53±0.57,P<0.01?compared with the the HO-1 over expression group.Pathological score of lung tissue in the HO-1 over expression group was significantly reduced?5.53±0.57 vs 9.37±0.61,P<0.05?compared with the SAP model group.4.Immunohistochemistry test results of IL-10 in lung tissue:The expression of IL-10 in the control group was normal,and no significant pathological changes.The expression of IL-10 in model group increased and immunohistochemical staining was more obvious compared with the control group.The expression of IL-10 increased in the HO-1 inhibitor group compared with SAP model group and immunohistochemical staining was more obvious compared with SAP model group,but lighter than HO-1 overexpression group.The expression of IL-10 increased significantly in the HO-1 overexpression group and immunohistochemical staining was more obvious compared with SAP model group.5.Immunohistochemistry test results of TNF-a in lung tissue:The expression of TNF-a in the control group was normal,and no significant pathological changes.The expression of TNF-a in model group increased significantly and immuno-histochemical staining was more obvious compared with the control group.The expression of TNF-a increased significantly in the HO-1 inhibitor group compared with SAP model group and immunohistochemical staining was more obvious compared with SAP model group and HO-1 overexpression group.The expression of TNF-a increased in the HO-1 overexpression group and immunohistochemical staining was lighter compared with SAP model group.Key proteins expression of p38 MAPK/NF-?B pathway1.Key proteins expression of p38 MAPK/NF-?B pathway in pancreatic tissues of rats in each group:Western Blot assay showed that HO-1 inhibitor could increase the phosphorylation of p38 and the expression of MAPKAPK2 in pancreatic tissue,and promote the expression of NF-?B p65.HO-1 overexpression could inhibit the phosphorylation of p38 and the expression of MAPKAPK2 and reduce the expression of NF-?B p65 in pancreatic tissue of SAP rats.2.Key proteins expression level of p38 MAPK/NF-?B pathway in lung tissue of rats in each group:western Blot results showed that HO-1 inhibitor could increase the phosphorylation of p38 and the expression of MAPKAPK2 and promote the expression of NF-?B p65 in lung tissue of SAP rats.HO-1 overexpression could inhibit the phosphorylation of p38 and the expression of MAPKAPK2 and reduce the expression of NF-?B p65 in lung tissue of SAP rats.Western Blot results suggest that p38 MAPK/NF-?B pathway is the key inflammatory signaling pathway for HO-1 to increase IL-10,reduce TNF-a and alleviate excessive inflammatory response in SAP rats.CONCLUSIONS:HO-1 overexpression can increase serum content of HO-1,IL-10,reduce the serum content of TNF-a,and increase the content of IL-10 and HO-1,reduce the content of TNF-a in pancreas and lung tissue,also can reduce the pathological score of pancreatic and lung tissue.Inhibition of HO-1 expression can reduce serum content of HO-1,IL-10,increase the content of TNF-a,and reduce the contents of IL-10,HO-1,increase the content of TNF-a in pancreas and lung tissue,also can increase the histopathological score of pancreatic and lung tissue.Research shows that:1.HO-1 overexpression can relieve inflammatory reaction in pancreas and lung tissue of SAP rats,and has protective effect on pancreas and lung tissue of SAP rats.2.The protective effect mechanism of HO-1 on the pancreas and lung of SAP rats may be related to the increase of IL-10 expression and the decrease of TNF-a expression.3.HO-1 overexpression can inhibit the phosphorylation of p38 and MAPK,and reduce the expression of NF-?B p65 in pancreas and lung tissues of SAP rats.4.The experimental results suggest that p38 MAPK/NF-?B pathway is the key inflammatory signaling pathway for HO-1 to increase IL-10,reduce TNF-a and alleviate excessive inflammation in SAP rats.BackgroundClinical treatment of severe acute pancreatitis includes conservative treatment or surgical treatment.A large number of clinical research has confirmed that surgical treatment can aggravate pancreatic and systemic inflammation because of surgical trauma and stress,and can lead to secondary severe infection which would cause multiple organ dysfunction syndrome such as lung,liver,kidney,heart or other organs dysfunction.In recent years,the incidence of severe acute pancreatitis in China is increasing,but there are lack of the effective treatments to improve the cure rate and survival rate.So the effective treatment of severe acute pancreatitis is a difficult international clinical problems.Traditional Chinese medicine is a treasure of Chinese traditional culture.Combination treatment of traditional Chinese and Western medicine is the characteristics and advantages of our country.Chinese medicine treatment of SAP has a unique curative effect and has become an important part of the SAP clinical treatment program.Dachaihu Decoction of the Han Dynasty's doctor Zhang Zhongjing is the classic prescription applied in the clinical treatment of pancreatitis which has beneficial clinical effect.Dachaihu Decoction commonly used in the clinical treatment of acute pancreatitis,gastric and duodenal ulcer,cholelithiasis,acute cholecystitis.In recent years,clinical studies and animal experiments showed that Dachaihu Decoction has a variety of pharmacological effects that can inhibit gastric acid hypersecretion to protect gastric mucosa,relaxes the sphincter tone,has choleretic effect,and protect the liver cell function.The clinical effect of Dachaihu Decoction in the treatment of SAP is worth looking forward to.But the mechanism is still not clear and need experimental study to be confirmed so that will provide experimental evidence for the clinical application.TNF-a and IL-10 are important pro-inflammatory and anti-inflammatory cytokines.IL-10 can inhibit the release of IL-2,IL-3,TNF-a and other cytokines,can also relieve inflammatory reaction and protect organ function.IL-10 has the potential anti-inflammatory function and protective effect on multiple organ injury caused by SAP.TNF-a is a cytokine released by activated macrophages and lymphocytes which can increased the expression of IL-6 and IL-8 which leading to cell damage and tissue destruction.Mitogen activated protein kinase?MAPK?inflammation signal transduction pathway has been proved to be the key way to induce HO-1 gene expression.However,the regulation of p38 MAPK/NF-kB inflammatory signal transduction pathway activated by HO-1 has not been reported at home and abroad.In the first part of SAP animal experiments,it has been confirmed that HO-1 can promote the expression of anti-inflammatory factor IL-10,reduce the expression of pro-inflammatory factor TNF-?,and has organ protective effect on pancreas and lung.HO-1 regulates p38 MAPK/NF-? B inflammatory signal transduction pathway to inhibit inflammatory response and organ protection.If Dachaihu decoction can inhibit the systemic inflammatory response of p38 MAPK/NF-? B by regulating HO-1,it will provide a new therapeutic target for the clinical treatment of SAP.The clinical effect of Dachaihu Decoction in the treatment of SAP and its mechanism still need experimental study to be confirmed.This experiment was designsd to tset the clinical effect of Dachaihu Decoction in the treatment of SAP.SAP rat models were treated with Dachaihu Decoction to observe inflammatory factor HO-1?TNF-a and IL-10 content in rat serum,pancreas and lung tissue,and to observe the pathological changes on pancreas and lung tissue.To observe the regulatory effect of Dachaihu Decoction on p38 MAPK/NF-?B inflammatory signal transduction pathway in pancreas and lung tissue of SAP rats,and to analyze whether Dachaihu Decoction has anti-inflammatory effect and organ protection by mediating HO-1/p38 MAPK/NF-?B inflammatory signal transduction pathway in SAP rats.If Dachaihu Decoction can block the excessive activation of systemic inflammatory response,it will provide a powerful weapon for the clinical treatment of SAP and has important clinical significance.OBJECTIVE:To observe the effect of Dachaihu Decoction on HO-1?TNF-a?IL-10 content of serum,pancreas and lung tissue,and pathological changes of pancreatic and lung tissue in rats with severe acute pancreatitis?SAP?.To explore the protective effect of Dachaihu Decoction in pancreas and lung inflammatory reaction.To observe the regulatory effect of Dachaihu Decoction on p38 MAPK/NF-?B inflammatory signal transduction pathway in pancreas and lung tissue of SAP rats,and to analyze whether Dachaihu Decoction has anti-inflammatory effect and organ protection by mediating HO-1/p38 MAPK/NF-?B inflammatory signal transduction pathway in SAP rats.METHODS:45 male SD rats?6 to 7 weeks old,weight of 220-260g?were randomly divided into 3 groups:control group,SAP model group,Dachaihu Decoction group,and 15 rats in each group.1.The control group:sham operation group,only open the abdominal cavity,and then closed.2ml normal saline gavage BID after operation,free drinking water.2.The SAP model group:the SAP model was established by retrograde pancreatic duct injection of 5%sodium taurocholate?0.1ml/100g?.2ml normal saline gavage BID after operation,free drinking water.3.The Dachaihu Decoction group:the SAP model was established by retrograde pancreatic duct injection of 5%sodium taurocholate?0.1ml/100g?,free drinking water after operation.The rats were given Dachaihu Decoction gavage?2ml/200g?BID,dose calculated by formula)after the SAP model was established.Dachaihutang formula:bupleurum 15g,scutellaria root 9g,peony 9g,pinellia 9g,citrus aurantium 9g,rhubarb 6g,ginger 15g,jujube 5 pieces?about 10g?,total weight about 82g.Dachaihu Decoction was decoctsd by traditional Chinese medicine room of the hospital affiliated to Shandong University of Traditional Chinese Medicine.82g Dachaihu Decoction was boiled into 200ml liquor.The rats were given 2ml Dachaihu Decoction gavage?calculated with 200g as the standard weight?,two times a day.Blood samples were collected from abdominal aorta 72hs after the model was made.Then rats were sacrificed,pancreas and lung tissue samples were collected.Pancreatic and lung tissue paraffin sections were prepared.To observe the pathological changes of pancreatic and lung tissue by HE staining and immunohistochemical staining.The contents of serum IL-10 and TNF-a were detected by ELISA method.The contents of IL-10 and TNF-a of pancreas and lung tissue homogenate were detected by ELISA method.Total proteins were extracted from pancreas and lung tissues.Western Blot method was used to detect the expression levels of key proteins of p38 MAPK/NF-?B pathway?p38 MAPK,phosphorylated P38 MAPK,MAPKAPK2,and NF-?B p65?.RESULTS:??Serum contents of HO-1?IL-10 and TNF-a of each group1.Comparison of serum HO-1:Compared with the control group,the serum contents of HO-1 in the SAP group were increased?0.8210.17 vs 0.26±0.05,P<0.01?.Compared with the SAP model group,the serum contents of IL-10 were significantly increased in the Dachaihu Decoction group?0.99±0.15 vs 0.82±0.17,P<0.05?.2.Comparison of serum IL-10:Compared with the control group,the serum contents ofIL-10 in the SAP group were elevated(74.62±7.28 vs 12.37±1.94,P<0.01.Compared with the SAP model group,the serum contents of IL-10 were significantly increased in the Dachaihu Decoction group?91.67± 10.53 vs 74.62±7.28.P<0.05?.3.Comparison of serum TNF-a:Compared with the control group,the serum contents of TNF-a in the SAP group were elevated?31.49±4.67 vs 11.73± 1.88,P<0.01?.Compared with the SAP model group,the serum contents of TNF-a were significantly decreased in the Dachaihu Decoction group?23.81 ±3.29 vs 31.49±4.67,P<0.05???Contents of HO-1?IL-10 and TNF-a in pancreatic tissue of each group1.Comparison of HO-1 in pancreatic tissue of each group:Compared with the control group,the HO-1 contents of pancreatic tissue in the SAP group were increased?0.49±0.07 vs 0.17±0.04,P<0.01?.Compared with the SAP model group,the HO-1 contents of pancreatic tissue were significantly increased in the Dachaihu Decoction group?0.78±0.14 vs 0.49±0.07,P<0.05?2.Comparison of IL-10 in pancreatic tissue of each group:Compared with the control group,the pancreatic tissue contents of IL-10 in the SAP group were elevated?50.37±4.56 vs 25.93±2.86,P<0.05?.Compared with the SAP model group,the pancreatic tissue contents of IL-10 were significantly increased in the Dachaihu Decoction group?59.82±6.35 vs 50.37±4.56,P<0.05?3.Comparison of TNF-a in pancreatic tissue of each group:Compared with the control group,the pancreatic tissue contents of TNF-a in the SAP group were elevated?31.76±5.52 vs 14.78±2.39,P<0.05?.Compared with the SAP model group,the pancreatic tissue contents of TNF-a were significantly decreased in the Dachaihu Decoction group?25.68±3.49 vs 31.76±5.52,P<0.05???Contents of HO-1?IL-10 and TNF-a in lung tissue of each group1.Comparison of HO-1 in lung tissue of each group:Compared with the control group,the HO-1 contents of lung tissue in the SAP group were increased?0.42±0.07 vs 0.13±0.02,P<0.01?.Compared with the SAP model group,the HO-1 contents of lung tissue were significantly increased in the Dachaihu Decoction group?0.65±0.09 vs 0.42±0.07,P<0.05?.2.Comparison of IL-10 in lung tissue of each group:Compared with the control group,the lung tissue contents of IL-10 in the SAP group were elevated?48.51 ±3.94 vs 20.63±1.89,P<0.05?.Compared with the SAP model group,the lung tissue contents of IL-10 were significantly increased in the Dachaihu Decoction group?57.26±6.07 vs 48.51 ±3.94,P<0.05?3.Comparison of TNF-a in lung tissue of each group:Compared with the control group,the TNF-a contents of lung tissue in the SAP group were elevated?28.43±2.92 vs 10.67±0.94,P<0.05?.Compared with the SAP model group,the TNF-a contents of lung tissue were significantly decreased in the Dachaihu Decoction group?10.67±0.94 vs 28.43±2.92,P<0.05???Morphology observation of pancreas:1.Gross specimen observation of pancreas;Pancreatic tissue of control group no edema,hyperemia,pancreatic necrotic,and no remarkable pathological changes;Pancreatic tissue of SAP model group was edematous,pale,and adipose tissue scattered punctate saponifying spots;Pancreatic tissue of Dachaihu Decoction group was edematous,pale and no obvious saponifying spots2.Microscopic observation:In control group,the pancreas acinar arrangement was rule,glandular morphology was normal,and there is no obvious pathologic changes.In the SAP model group,pancreatic interstitial was congestive and edematous,accompanied by inflammatory cell infiltrated;Part of the pancreas was congestive,necrotic,and alveolar arranged in disorder.In Dachaihu Decoction group,pancreatic interstitial was edematous,small amount of inflammatory cell infiltrated;Pathological changes were lighter compared with the SAP model group3.Pathological score of pancreatic tissue:Pathological score of pancreatic tissue in the Dachaihu Decoction group was significantly reduced?8.50±0.78 vs 12.30±1.26,P<0.05?compared with the SAP model group.Pathological score of pancreatic tissue in the SAP model group was significantly increased?12.30± 1.26 vs 0,P<0.01?compared with the control group.4.Immunohistochemistry test results of IL-10 in pancreatic tissue:The expression of IL-10 in the control group was normal,and no significant pathological changes.The expression of IL-10 in model group increased and immunohistochemical staining was more obvious compared with the control group.The expression of IL-10 increased significantly in the Dachaihu Decoction group and immunohistochemical staining was more obvious compared with SAP model group5.Immunohistochemistry test results of TNF-?in pancreatic tissue:The expression of TNF-a in the control group was normal,and no significant pathological changes.The expression of TNF-? in model group increased significantly and immunohistochemical staining was more obvious compared with the control group.The expression of TNF-? increased in the Dachaihu Decoction group and immunohistochemical staining was lighter compared with SAP model group.??pathological observation of lung tissue:1.Gross specimen observation of lung:Lung tissue of control group was no edema,hyperemia and no remarkable pathological changes.Lung tissue of SAP model group was edematous,pale.Lung tissue of Dachaihu Decoction group was edematous,pale and pathologic changes was lighter compared with SAP model group.2.Microscopic observation:In control group,the structure of lung tissue was complete,clear and the cell arrangement was rule.There was no inflammatory cell infiltration and no congestion,edema,alveolar.There is no obviously pathologic changes.In the SAP model group,the structure of lung tissue was fuzzy,a large number of inflammatory cells infiltrated,a small amount of red blood cell effusion,and alveolar wall was thickening.Interstitial of lung tissue was congestive and edematous,accompanied by inflammatory cell infiltrated.In Dachaihu Decoction group,the structure of lung tissue was complete,a large number of inflammatory cells infiltrated,a small amount of red blood cell effusion.Pathological changes were lighter compared with the SAP model group.3.Pathological score of lung tissue:Pathological score of lung tissue in the Dachaihu Decoction group was significantly reduced?5.35?0.49 vs 8.93±0.85,P<0.05?compared with the SAP model group.4.Immunohistochemistry test results of IL-10 in lung tissue:The expression of IL-10 in the control group was normal,and no significant pathological changes.The expression of IL-10 in model group increased and immunohistochemical staining was more obvious compared with the control group.The expression of IL-10 increased significantly in the Dachaihu Decoction group and immunohistochemical staining was more obvious compared with SAP model group.5.Immunohistochemistry test results of TNF-ain lung tissue:The expression of TNF-a in the control group was normal,and no significant pathological changes.The expression of TNF-a in model group increased significantly and immunohistochemical staining was more obvious compared with the control group The expression of TNF-a increased in the Dachaihu Decoction group and immunohistochemical staining was lighter compared with SAP model group?.Expression of key proteins of p38 MAPK/NF-?B pathway in pancreatic tiss... |
PDF Full Text Request