Analysis Of Multiphoton Microscopic Characteristics For Brain Tumor

Brain tumor is a common neurological disease that accounts for 20% of the whole-body tumors.No matter it is benign or malignant,the mass effect can cause damage to brain functions,or even threaten life.The treatment options for different tumors or different grades of the same tumor will vary.Therefore,the preoperative and intraoperative localization and qualitative diagnosis,as well as differential diagnosis are particularly important for the success of treatment and the prognosis of patients.However,the resolution of current clinical diagnostic techniques is generally based on "mm",so it is impossible to assess the pathological state of brain diseases at the cellular level.Therefore,it is of great significant to explore new in situ non-destructive diagnosis methods with histopathology resolution for the diagnosis and identification of brain tumors.In recent years,multiphoton microscopy technology has presented great feasibility and broad application prospects in tumor research,which has the advantages of high spatial resolution,high sensitivity,low damage to biological tissues and deep imaging depth,etc.Therefore,the biological tissue can be analyzed horizontally at the subcellular level to obtain its microstructure and spectral information.In this paper,we mainly carry out brain tumor research based on multiphoton microscopy technology.Firstly,the multiphoton microscopy technology is applied to study glioma.The results showed that(1)the optical diagnostic features of gliomas can be established.(2)combined with image processing,the microenvironment and metabolism of glioma can be qualitatively and quantitatively analyzed simultaneously.(3)combined with image processing,the blood vessel in gliomas can be qualitatively and quantitatively analyzed.(4)combined with image processing,low-grade and high-grade gliomas can be automatically distinguished.Secondly,the multiphoton microscopy technology is adopted to image meningioma.The results showed that(1)the multiphoton microscopy can not only identify meningioma,but also distinguish the pathological types of meningioma(epithelial meningioma,fibrous meningioma,angiomatous meningioma,transitional meningioma,psammomtous meningioma).(2)multiphoton microscopy can also detect the braininvasion in meningioma.Thirdly,multiphoton microscopy imaging on other brain tumors are also conducted.(1)combined with image analysis,some qualitative optical diagnostic features and quantitative diagnostic parameters of lymphoma and germinoma can be obtained.(2)combined with image analysis,qualitative optical diagnostic features and quantitative diagnostic parameters of schwannoma can be obtained.(3)combined with image analysis,the texture of pituitary adenomas can be qualitatively and quantitatively analyzed.In summary,through the research work of this thesis,the research of brain tumor based on multiphoton microscopy technology has been carried out.Some innovative results have been obtained,which the groundwork for the further use of MPM to perform a real-time noninvasive diagnosis of brain tumor in clinic.