Development of methods to identify RNAs with 2'3' cyclic phosphate termini and to determine of genome architecture in Saccharomyces cerevisiae

The development and application of novel molecular technologies is necessary to better understand fundamental processes in molecular and cellular biology. For example, next-generation sequencing technologies have furthered our analysis of genetic variation and transcriptional regulation. I describe two novel technologies that take advantage of next-generation sequencing technology. The first is a method that employs tRNA ligase from Arabidopsis thaliana to isolate RNAs with 2'3' cyclic phosphate termini which are identified by sequencing of their cDNAs. The second technology is the result of a collaborative effort that adapts the chromosome conformation capture method to a large scale in order to analyze the nuclear architecture of the Saccharomyces cerevisiae genome.