Cloning and sequence analysis of multiple genes from Bifidobacterium infantis

Bifidobacteria are probiotics that are commonly added in a multitude of dairy products. However, there is a lack of knowledge of their genes. Investigations into the multiple genes in this important organism will contribute to our understanding and ability to manipulate it for future important commercial application purposes. Our objectives were to clone and sequence multiple genes from Bifidobacterium infantis.; A genomic DNA library of B. infantis 15702 was constructed in lambda phage and 5 positive clones were further analyzed. Sequencing was performed by primer walking from both of the 5' and 3' directions of the cloned DNA.; Sequence results revealed the presence of six open reading frames (ORF) on the cloned fragments. The six ORF had the highest identities with the epimerase of Streptococcus mutans (60.8%), beta-galactosidase of Leuconostoc lactis (strain NZ6009) plasmid pNZ63 (62.5%), lipase of Deinococcus radiodurans (strain R1) (34.2%), 50S ribosomal proteins L9 of section 6 of 114 of the complete genome of Rickettsi conorii Malish 7 (59.9%), transposase of Deinococcus radiodurans (strain R1) (35.0%), and aminoglycoside N3-acetyltransferases of Nostoc sp. (strain PCC 7120) (39.1%), respectively.; The structure of the bifidobacterial genes will provide the basis for genetic approaches that will lead to potential developments of commercial strains with improved characteristics.