Inhibitory potencies of several non-steroidal anti-inflammatory drugs against sulfotransferase 1A1

Non-steroidal anti-inflammatory drugs (NSAIDs) are among the most prescribed medications worldwide. Their elimination from the body is complex and partly depends on metabolizing enzymes which influence duration of action and half-life. One of the metabolizing enzyme families is sulfotransferases (SULT), a group of enzymes with the capability to modify the half-life of NSAIDs. Among all sulfotransferases in the liver, SULT1A1 has the most activity for NSAIDs sulfation. The goal of this investigation was to study the inhibition of five NSAIDs on the human liver SULT1A1 activity. We used both recombinant and pooled cytosolic human SULT1A1 to screen the inhibitory effects of five NSAIDs. As a positive control we used mefenamic acid. We concluded that at the therapeutic doses, none of the tested NSAIDs has the potential to significantly inhibit the activity of liver SULT1A1.