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A development of rapid methods for detecting food-borne pathogens by using immunochemical techniques

Posted on:1999-12-18Degree:Ph.DType:Dissertation
University:University of GeorgiaCandidate:Seo, KunhoFull Text:PDF
GTID:1461390014967487Subject:Agriculture
Abstract/Summary:
rapid detection method for Escherichia coli O157:H7 combining immunomagnetic beads (IMB) and flow cytometry was developed and investigated. Immunomagnetic separation and flow cytometry (IMFC) enabled the detection of ;The developed IMFC assay was evaluated to detect low number of E. coli O157:H7 spiked in ground beef, applejuice, and raw milk. IMFC detected 10;An integrated optic interferometer for detecting food-borne pathogens has been developed. Salmonella typhimurium was detected by binding to antibody against Salmonella common structural antigen immobilized on a silane-derivatized sensor surface at concentrations in the range ca. 1...
Keywords/Search Tags:Detecting food-borne pathogens, Coli o157, Flow cytometry
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