Analysis of substrate analogs for 4-HBA-CoA thioesterase indicated that the hydroxylbenzoyl group plays and the nucleotide portion of the CoA moiety function in substrate binding and recognition Analysis of the k;The substrate specificity of 4-CBA-CoA dehalogenase is shown to be governed by the sizes of the para substituent on the benzoyl ring, and the conformation of the substrate/product analog free in solution. UV-visible, ;Catalytic residues functioning in the dehalogenase enzyme active site were probed using chemical modification and site-directed mutagenesis techniques. Three residues, Trp137, and His90 and His81, were identified. UV-vis and... |