| roper glycoprotein processing is essential to all known eukaryotes. In fact, numerous human diseases are due to the improper processing and trafficking of glycoproteins. This work examined the glycoprotein processing in the secretory pathway of Schizosaccharomyces pombe, a fission yeast with many evolutionarily-conserved similarities to mammalian cells. Chemical structures of the oligosaccharides of secreted glycoproteins from S. pombe have been defined by separately removing the N- and O-linked oligosaccharides from glycoproteins by enzymatic hydrolysis and... |
