LASER SPECTROSCOPY OF BIOLOGICAL MACROMOLECULES. I. DYNAMIC LIGHT SCATTERING OF POLY-L-GLUTAMIC ACID. II. OPTOACOUSTIC SPECTROSCOPY OF AQUEOUS HEMOGLOBIN |
Posted on:1981-05-06 | Degree:Ph.D | Type:Dissertation |
University:The Pennsylvania State University | Candidate:BERGER, ELISABETH JANE | Full Text:PDF |
GTID:1471390017966551 | Subject:Chemistry |
Abstract/Summary: | |
Two areas of laser spectroscopy, dynamic light scattering and optoacoustic spectroscopy, have been investigated. Dynamic light scattering was used to investigate the pH-induced (alpha)-helix-random coil transition in poly-L-glutamic acid. By measuring the translational-diffusion coefficient the transition was monitored as a function of pH. A theoretical investigation was made of the possibility of using DLS to determine the kinetics of the transition. It was determined that this is not possible.; Optoacoustic Spectroscopy was used as an analytical technique to detect human hemoglobin in aqueous solution in nonomolar concentrations. A pulsed laser system was used to excite the solute, and a piezoelectric transducer was used to detect the acoustic waves. |
Keywords/Search Tags: | Dynamic light scattering, Optoacoustic spectroscopy, Laser, Used |
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