Effects And Mechanism Of Rhynchophylla Total Alkaloid Inhibiting Vascula Remondeling In Adventitia

Objective:To discuss the effects and mechanism of rhynchophylla total alkaloid inhibiting vascular remondeling in adventitia. And the mechanism inhibiting the proliferation of vascular adventitia fibroblast. Methods:Based on vascular adventitia fibroblast culture, we model hypertension vascular remondeling by angiotension?;Obverving ryhnehophylla total alkaloid cytotoxicity and choosing appropriate drug concentration, we make use of MTT; Using electron microscope, we can visually observe proliferous vascular adventitia fibroblast induced by angiotension?;Making use of flow cytometry, we can research the variety of vascular adventitia fibroblast's cell cycle affected by rhynchophylla total alkaloid; We also test the gene expression of collagen type?and?. Results:It is successful for us to model hypertension vascular remondeling by angiotension?.And the most appropriate concentration for angiotension?is 2×10-7mol/L. 200ug/ml⁴00ug/ml rhynchophylla total alkaloid inhibiting is the best drug concentration.Rhynchophylla total alkaloid can inhibit the proliferation of vascular adventitia fibroblast from MTT and electron microscope. The results of low cytometry show that rhynchophylla total alkaloid can increase VAF in G₀ /G₁ phase and decrease VAF in S phase. And this inhibition is dependent on drug concentration. Rhynchophylla total alkaloid can inhibit the gene expression of collagen type?and?. Conclusions: Rhynchophylla total alkaloid can inhibit proliferation of vascular adventitia fibroblast. The mechanism of this inhibiting effect probably in two ways. The one is to increase VAF in G0 /G1 phase and decrease VAF in S phase. The other is to inhibit the gene expression of collagen type?and?.So rhynchophylla total alkaloid can inhibit vascular remondeling in adventitia.