| Objective: to explore the mechanism of electro-acupuncture analgesia on CFA-induced arthritis inflammatory pain model (AA rats);To explore the the relation of CRH with 5-HT or?-EP on the effect of acupuncture analgesia through the way of injecting anti-corticotropin-releasing-hormone serum (anti-serum of CRH) and anti-5-HT serum or anti?-EP serum from the lateral ventricle of cerebrum on AA rats; to explore the mechanism of G-protein mediated signal transduction of CRH in pain and acupuncture analgesia by studying the changes of the expression of inhibitory GTP-binding protein (Gi) in hypothalamus after acupuncture or injecting anti-serum of CRH from the lateral ventricle of cerebrum on AA rats.Methods: AA rats were used as the subjects of the study. By using methods of behavioral threshold of pain test, measurement of foot cubage,high performance liquid chromatographic(HPLC) and radioimmunoassay et al, we observed the influence of EA at EX-B2 on AA rats and injection of anti serum of CRH from the threshold of pain, foot cubage, the level of IL-1? and TNF-?in blood serum, the content of 5-HT and?-endorphin in hypothalamus or spinal cord. Observe the changes of pain threshold after inject anti-serum of 5-HT or?-endorphin on anti-serum of CRH or electro-acupuncture.The expression of NF-?B or CRH and Gi in hypothalamus was also observed by using methods of immunohistochemistry and western blot.Results: The pain threshold of AA rats were improved and the foot cubage foot swelling rate were reduced by using electro-acupuncture at EX-B2. The amounts of 5-HT and?-EP in hypothalamus and spinal cord increased after electro-acupuncture while the IL-1? and TNF-?decreased in blood serum. We also observed that the expression of NF- ?B in hypothalamus decreased remarkably after electro-acupuncture. And the expression of CRH increased .However,after injection of anti-serum of CRH weakened the pain threshold of AA rats and the effect of acupuncture analgesia. the amounts of 5-HT and?-EP and the expression of CRH in hypothalamus decreased. If add injection the anti-serum of 5-HT ,the pain threshold mostly decreased than before.but when add injection the anti-serum of?-EP ,no difference on the pain threshold happened,and the expression of CRH increased than before.from the results of western -blot we can see that the inhibitory G-protein in hypothalamus decreased after electro-acupuncture while increased after inject the anti-serum of CRH .Conclusion: the mechanism of electro-acupuncture analgesia and the function of immune regulation may be related with the activity of NF-?B.the mechanism of acupuncture analgesia and regulation of NEI may be related with the G-protein mediated signal transduction pathway.The endogenous of CRH may be related with the 5-HT or?-EP during the process of electro-acupuncture analgesia.The endogenous of CRH may be related with the G-protein mediated signal transduction pathway during the process of electro-acupuncture analgesia. |
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