| Part one experimental study:To explore the effect of Qingre jiedu on AS based on autophagy-related signaling pathwayOBJECTIVE:On the basis of the previous period,the New Zealand rabbit AS model was constructed to study the effects of Qingrejiedu herbs on AS plaques,inflammatory factors,apoptosis,and autophagy and their related signaling pathways,so as to explore whether Qingrejiedu traditional Chinese medicines play a role in stabilizing the plaque by regulating autophagy and explore its specific molecular mechanisms.Methods:Seventy New Zealand male rabbits,seven days after adaptive feeding,were randomly divided into seven groups(n=10)including blank group,model group,Huanglian Jiedu Tang low,middle and high dose groups,chloroquine group and Lipitor group.The normal control group was fed normal feed(120-140 g/d for 16 weeks),and the remaining groups were fed high-fat diet(2%cholesterol,2%lard,94%normal feed,120-140 g/d for 16 weeks),and in the the fourth week,eighth week,and twelfth weeks,LPS was injected intramuscularly in the right ear artery,carotid artery and superficial femoral artery(intramuscular injection of lug for each site,dissolved in 0.1 mL of water for injection,each Each rabbit was injected with 3ug LPS)。From the eighth week,Huanglian Jiedu Decoction low-dose group,Huanglian Jiedu Decoction middle-dose group and Huanglian Jiedu Decoction high-dose group began to take Huanglian Jiedu Tang 0.75g/kg/d,1.5g/kg/d and 3g/kg/d gavage,atorvastatin group was administered at a dose of 1 mg/kg/d of atorvastatin。In the chloroquine group,chloroquine(autophagy inhibitor,50 mg/kg/d,the effect was to increase the lysosome’s internal pH and block the fusion of lysosomes and autophagosomes,thus blocking Autophagic process)was given by intraperitoneal injection at the same time as high-dose Huanglian Jiedu Decoction for eight weeks.At the end of the sixteenth week,all rabbits were received a drug to induce plaque rupture(histamine injection at a dose of 0.02 mg/kg)and IVUS was used to observe aortic plaque morphology before and after drug induction.Twenty four hours after drug induction,all animals were killed and the following indicators were tested:1.Biochemical assays were used to detect blood lipid levels(TC,TG,LDL-C,HDL-C).Serum LPS levels were measured by limulus test.Serum CRP and IL-1 levels were measured by ELISA.2.Aortic plaque stability analysis:①HE staining was performed to observe the incidence of plaque rupture.②IVUS was used to measure the area of elastic membrane(EEMA),lumen area(LA),plaque area(PA)and plaque load(PB);③Immunohistochemical method(IH)was used to determine the content of macrophage and smooth muscle(a-SMA)cells in plaques.Picrosirius red staining was used to determine the content of collagen fibers in plaques.Oil red 0 staining was used to determine the lipid content in plaques.,to calculate the plaque’s instability index(Ⅵ):VI=(macrophage staining%+lipid staining%)/(smooth muscle cell%+collagen fiber%).3.Inflammation level evaluation:IH staining was used to determine the expression of MMP9 protein in plaques.The macrophage apoptosis and necrosis were assessed by TUNEL staining.The apoptosis of macrophages in plaques was detected.The expression of apoptotic protein Caspase-3 in plaques was determined by immunofluorescence.HE staining measures the degree of cell necrosis within the plaque.Macrophage Evaluation of Apoptotic Cell Scavengability:Observation of the Number of Macrophages(F4/80 Markers)Associated with Apoptotic Cells(TUNEL Positive)in the Plaque,and the Number of Free Macrophages(F4/80 Markers)The ratios were evaluated for macrophage scavenging apoptotic macrophages.4.Evaluation of autophagy:① Observe the autophagosomes in macrophages using transmission electron microscope;②Determine the expression of LC3-Ⅱ by immunofluorescence;③IH assay the expression of Atg5-Atg12 complex and p62/SQSTM1.Detection of autophagy regulation protein:IH assay was used to detect the expression of mTOR,Akt and AMPK.results:1.Huanglian Jiedu Decoction and Lipitor all can down the serum TG,LDL-C,TC,increased HDL,and Chinese medicine high-dose group and the Lipitor group is better than the medium dose and low dose group and chloroquine group P<0.05),Huanglian Jiedu Decoction high-dose group and Lipitor group no significant difference(P>0.05).2.Both Huanglian Jiedu Decoction and Lipitor can reduce serum CRP,LPS,IL-1 levels,CRP level in CRP is lower than traditional Chinese medicine dose group and Lipitor group(P<0.05)(P<0.05).The CRP level of middle dose group and chloroquine group was higher than that of traditional Chinese medicine high dose group(P<0.05).The CRP level of chloroquine group was higher than that of Lipitor group(P<0.05).In LPS,the LPS level of Chinese medicine low dose group and traditional Chinese medicine middle dose group was higher than that of middle and high group(P<0.05);Chinese medicine high dose group was lower than chloroquine group and Lipitor group(P<0.05).Compared with model group,IL-1 level in each intervention group decreased significantly(P<0.05)in IL-1 group;IL-1 level in low dose group was higher than that in high dose group and Lipitor group(P<0.05).The levels of IL-1 in middle-dose group,high-dose group and Lipitor group were lower than those in chloroquine group(P<0.05).3.HE staining results:① blank group:the aortic intimal layer,single layer of flat cells,no proliferation;middle layer smooth muscle and elastic fibers go consistent,no obvious abnormalities.② Model group:The aortic intima was evenly thickened,and the thickened plaque was mainly composed of foam cells,collagen,depositional lipids,a small amount of necrotic substances,and a small amount of cholesterol crystals;basically no obvious fibrous caps were found;thick.③ Chinese medicine low-dose group:The aortic intima is thick and uneven,and the plaque is mainly composed of a large number of fibrocytes,some foam cells and a small amount of inflammatory cells;the middle membrane structure is disordered,and multiple necrotic calcifications can be seen.④ medium dose group:moderate thickening of the aortic intima,plaques with varying degrees of calcification and necrosis,some damage to the infiltration of the middle layer,smooth muscle and elastic fiber basophilic enhancement,partial necrosis calcification.⑤ High-dose traditional Chinese medicine group:locally thickened aortic intima,necrotic calcification of hyperplastic plaques,disordered arrangement of smooth muscle and elastic fibers in the middle tunica media,and calcification of multiple focal necrosis.Chloroquine group:The aortic intima protrudes into the lumen,the lumen is narrow,and the intima is obviously thickened with a thin layer of fibrous caps.A long strip of calcification is visible in the arterial plaque.The infiltration of inflammatory cells,plaques can be seen in a large number of foam cells,smooth muscle cells in the middle layer of foam changes occurred.⑦ Lipitor group:there was a small amount of small fatty plaques in the aortic intima.There was no obvious stenosis in the lumen.A small amount of foam cells were seen in the plaque.No obvious fibrous cap was seen.No inflammatory cell infiltration was seen in the local media.No obvious thickening of the arterial wall.4.IVUS test:①There was no statistical difference between each groups on EEMA(P>0.05);②In LA,the model group had a decrease compared with the blank group(P<0.05).When each intervention group were compared with the model group,the Huanglian Jiedu decoction high dose group and Lipitor group were significantly increased.The difference was statistically significant(P<0.05);comparation between each intervention group:Huanglian Jiedu decoction high-dose group and Lipitor group were significantly higher than Huanglian Jiedu Decoction,low-dose group and chloroquine group,with significant differences(P<0.05),while the chloroquine group had lower levels than the Huanglian Jiedu Tang middle and low dose group,the difference was statistically significant(P<0.05);however,there was no significant difference between Huanglian Jiedu decoction low dose group and Huanglian Jiedu decoction middle dose group There was no significant difference between the Huanglian Jiedu decoction high-dose group and Lipitor group(P>0.05).③In PA,the model group was significantly larger than the blank group(P<0.05).When each intervention group were compared with the model group in,Huanglian Jiedu decoction low,medium,and high doses and Lipitor group were smatter than the model group.The difference was statistically significant(P<0.05).There was no significant difference between the chloroquine group and the model group(P>0.05).The comparison between each intervention group was that:Huanglian Jiedu decoction high-dose group and the Lipitor group were decreased significantly than that of Huanglian Jiedu decoction low-and middle-dose group(P<0.05),and the chloroquine group had an increase compared with Huanglian Jiedu decoction low dose group and medium dose group,and the difference was statistically significant(P<0.05).There was no significant difference between Huanglian Jiedu decoction low-dose group and Huanglian Jiedu decoction middle-dose group(P>0.05);meanwhile Huanglian Jiedu decoction high-dose group was significantly decreased than chloroquine group,the difference was statistically significant(P<0.05).however there was no significant difference between Huanglian Jiedu decoction high-dose group and Lipitor group,(P>0.05).④In PB,the model group was significantly increased compared with the blank group(P<0.05).Huanglian Jiedu decoction low,middle,and high dose groups and Lipitor group were significantly lower than the model group(P<0.05).The chloroquine group had a decreasing trend compared with the model group(P>0.05).The comparison between each intervention group was that:Huangtianjiedu decoction high-dose group and Lipitor was significantly decreased than Huanglian Jiedu decoction low-dose group and middle-dose group(P<0.05),and the chloroquine group had an increase when compared with Huanglian Jiedu decoction low-dose and middle-dose group(P<0.05),while there was no significant difference between Huanglian Jiedu decoction low-dose group and middle-dose group(P>0.05);Huangtian Jiedu decoction high-dose group was significantly decreased than chloroquine group,the difference was statistically significant(P<0.05).However,there was no significant difference between Huanglian Jiedu decoction high-dose group and Lipitor group(P>0.05).5.Plaque vulnerability index:The plaque vulnerability index of the model group was increased than that of the blank group(P<0.05);the plaque vulnerability index of each intervention group was decreased than that of the model group(P<0.05).Comparation between each intervention group was that:The plaque vulnerability index of Lipitor group、Huanglian Jiedu decoction middle-dose and high-dose group was decreased than that of Huanglian Jiedu decoction low-dose group of,the difference was statistically significant(P<0.05),while the plaque vulnerability index of the chloroquine group was increased,the difference was statistically significant(P<0.05);The plaque vulnerability index of Lipitor and Huanglian Jiedu Decoction high-dose group had a statistically significant decrease when compared with the Huanglian Jiedu decoction middle-dose group(P<0.05).The plaque vulnerability index in chloroquine group was increased than that of Huanglian Jiedu decoction middle-dose group,the difference was statistically significant(P<0.05).There was no significant difference between Huanglian Jiedu decoction high-dose group with Lipitor group,in plaque vulnerability index(P>0.05),however,the plaque vulnerability index of chloroquine group was increased significantly than that of Huanglian Jiedu decoction high-dose group(P<0.05);the plaque vulnerability index of Lipitor group was significantly decreased than that of chloroquine group(P<0.05).6.The expression of MMP9 in plaques:the level of MMP9 in plaques in the model group were higher than that in the blank group(P<0.05).The level of MMP9 in each intervention group was significantly decreased than the model group(P<0.05).Comparation between each intervention group was that,the MMP9 in the plaque of the Lipitor group、Huanglian Jiedu decoction middle-dose and high-dose group were lower than that in Huanglian Jiedu decoction low-dose group(P<0.05),while there was no significant difference between the chloroquine group and Huanglian Jiedu decoction low-dose group(P>0.05).When compared with the Huanglian Jiedu decoction middle-dose group,the level of MMP9 in chloroquine group was increased while the level of MMP9 in the plaques of Huanglian Jiedu decoction group and Lipitor group were significantly decreased(P<0.05).When compared with Huanglian Jiedu decoction high-dose group,the the level of MMP9 in chloroquine group was significantly increased(P<0.05),however there was no significant difference between Huanglian Jiedu decoction high-dose group and Lipitor group(P>0.05).7.Apoptosis was detected by TUNNEL staining in plaques:When compared with the blank group,the apoptosis of the model group increased significantly,the difference was statistically significant(P<0.05);the comparation between each intervention group and the model group was that:Each intervention group was significantly lower than the model group,the difference was statistically significant(P<0.05);the comparation between each intervention group was that:Lipitor group、Huanglian Jiedu decoction middle-dose and high-dose group was lower than that of Huanglian Jiedu decoction low-dose group(P<0.05).There was no significant difference between chloroquine group and Huanglian Jiedu decoction low-dose group(P>0.05).The Lipitor group and Huanglian Jiedu decoction high-dose group was significantly decreased than that of Huanglian Jiedu Decoction middle-dose group(P<0.05),and chloroquine group was increased than that of Huanglian Jiedu decoction middle-dose group(P<0.05);Huanglian Jiedu decoction high-dose group and Lipitor group wewe significantly lower than that of chloroquine group,(P<0.05),but there was no significant difference between Huanglian Jiedu decoction high-dose group and Lipitor group(P>0.05).8.Evaluation of Autophagy Level in Plaque:①In AMPK,there was no significant difference between the model group and the blank group(P>0.05).When compared with the model group,the AMPK levels in the plaques of all intervention groups were increased(P<0.05).The comparation between each intervention group was that:when compared with Huanglian Jiedu decoction low-dose group,the AMPK in the plaques of the Lipitor group、Huanglian Jiedu decoction middle-dose and high-dose groups were significantly higher(P<0.05),while chloroquine group was decreased(P<0.05);When compared with Huanglian Jiedu decoction middle-dose group,AMPK in the plaques in Huanglian Jiedu decoction high-dose group and Lipitor group was increased(P<0.05),however,chloroquine group was decreased(P<0.05);When compared with Huanglian Jiedu decoction high-dose group,the AMPK in the chloroquine group was decreased significantly(P<0.05),but there was no significant difference between the Lipitor group and Huanglian Jiedu decoction high-dose group(P>0.05).②In Akt:the level of Akt in the plaques of the model group was increased than that in blank group(P<0.05).When compared with model group,the level of Akt in the plaques of each intervention group was decreased(P<0.05).Comparation between each intervention group:when compared with Huanglian Jiedu decoction low-dose group,the Akt in the Lipitor group、Huanglian Jiedu Tang middle-dose and high-dose group was decreased(P<0.05),while the Akt level in chloroquine group was increased(P<0.05);when compared with the Huanglian Jiedu decoction middle-dose group,Lipitor and Huanglian Jiedu decoction high-dose group was decreased(P<0.05),however,the Akt level in chloroquine group was increased,the difference was statistically significant(P<0.05);when compared with Huanglian Jiedu decoction high-dose group,the Akt level in chloroquine group was increased(P<0.05),however,there was no statistical difference between the Lipitor group and Huanglian Jiedu decoction high-dose group(P>0.05).③In mTOR:the mTOR level in the plaques in the model group was increased than that of the blank group(P<0.05);Lipitor and Huanglian Jiedu decoction each dose group were decreased than model group,and the difference was statistically significant(P<0.05)while the mTOR level in chloroquine group showed a decreasing trend,although there was no statistical difference(P>0.05);comparation between each intervention group:when compared with the Huanglian Jiedu decoction low-dose group,the level of mTOR in the plaques of Lipitor group、Huanglian Jiedu decoction middle-dose and high-dose group were significantly lower(P<0.05),while the mTOR levels in the plaques in chloroquine group were significantly higher than those in Huanglian Jiedu decoction low-dose group(P<0.05).when compared with the Huanglian Jiedu decoction middle-dose group,the mTOR level in the plaques of Lipitor and Huanglian Jiedu decoction high-dose group were decreased(P<0.05),however the Akt level in chloroquine group was increased(P<0.05).when compared with Huanglian Jiedu high-dose decoction group,the mTOR level in the chloroquine group was increased(P<0.05),however,there was no difference between Lipitor group and Huanglian Jiedu high-dose decoction group(P>0.05).conclusion:1.Each dose of Huanglian Jiedu decoction and Lipitor can reduce LDL-C,TC,TG level and increase HDL level in model animals,meanwhile Huanglian Jiedu decoction showed dose-dependent.2.Huanglian Jiedu decoction each dose group and Lipitor can reduce the level of IL-1,CRP,LPS and inhibit inflammation.The effect of high-dose group is more obvious.Among them,Huanglian Jiedu Decoction high-dose group is superior to Lipitor in reducing LPS.3.Huanglian Jiedu decoction can antagonize the formation of AS plaques caused by high fat and other compound factors,which can protect the vascular intima,reduce the intimal hyperplasia,reduce the infiltration of foam cells,inflammatory cells,and reduce the deposition of lipids and cholesterol crystals.Huanglian Jiedu decoction play a role in plaque protecting and stabilizing the plaque and high-dose of Huanglian Jiedu decoction group has the most obvious effect,which shows dose-dependent,and the effect is equivalent to Lipitor.4.Huanglian Jiedu decoction and Lipitor can reduce the plaque area(PA)and plaque burden(PB)by different extents,and increase the lumen area(LA).Among them,Huanglian Jiedu decoction shows a dose-dependent effect,while Chloroquine seems to have some antagonism effect to Huanglian Jiedu decoction.5.Huanglian Jiedu decoction and Lipitor can reduce the plaque instability index(Ⅵ)and stabilize the plaque.The stability effect of the Huanglian Jiedu decoction is dose-dependent,and the higher the dose is,the better the plaque stabilization effect is.It was found that chloroquine may have some antagonistic effect on the stabilizing effect of Huanglian Jiedu decoction.Studies have showed that moderate autophagy is protective to the plaque.Therefore,we speculated that the stable plaque effect of Huanglian Jiedu decoction seems to be related to autophagy and needs further study.6.Huanglian Jiedu decoction and Lipitor have the effect of down-regulating MMP9 expression in plaques,and have certain anti-inflammatory and plaque stabilization effects.Among them,Huanglian Jiedu decoction high-dose group and Lipitor group down-regulated MMP9 expression most obviously,suggesting that Huanglian Jiedu decoction has a dose-dependent effect.At the same time,we noticed that chloroquine,an autophagy inhibitor,seems to weaken the effect of Huanglian Jiedu decoction in down-regulating MMP9 expression.7.All doses of Huanglian Jiedu decoction and Lipitor had the effect of inhibiting apoptosis.Huanglian Jiedu decoction was dose-dependent.The higher the dose was,the more obvious the effect of inhibiting apoptosis was,and even Huanglian Jiedu decoctionthe high-dose group was as effective in inhibiting apoptosis as Lipitor.However,the effect of Huanglian Jiedu decoction seems to be interfered by chloroquine.In the study,the apoptosis rate in chloroquine group(Huanglian Jiedu decoction high-dose gavage plus chloroquine intraperitoneal injection)was as high as in Huanglian Jiedu decoction low-dose group,which was higher than that in the Huanglian Jiedu decoction middle-dose and high-dose group,suggesting that chloroquine could antagonize the anti-apoptosis effect of Huanglian Jiedu decoction.8.Huanglian Jiedu decoction and Lipitor had some influence on autophagy signaling pathway,both of them could down-regulate Akt-mTOR expression and up-regulate AMPK expression.Among them,Huanglian Jiedu decoction high-dose group and Lipitor were significantly superior to Huanglian Jiedu decoction low-dose and middle-dose groups.However,there was no significant difference between Huanglian Jiedu decoction high-dose group and Lipitor group.At the same time,we observed that the Akt-mTOR level in the chloroquine group was significantly higher and the AMPK level was decreased,suggesting that chloroquine may have antagonistic effects to Huanglian Jiedu decoction in down-regulating Akt-mTOR and upregulating AMPK signaling pathway.9.The study found that intervention with Lipitor and Huanglian Jiedu decoction may increase autophagy by down-regulating Akt-mTOR signaling pathway and up-regulating AMPK signaling pathway,reducing macrophage apoptosis,inhibiting inflammatory response in plaque,and stabilizing AS plaques.At the same time Huanglian Jiedu decoction was dose-dependent.Part two clinical research:a research on the pattern of AM I syndromeObjective:The study collected patients who wree diagnosed as acute myocardial infarction(AMI)and were admitted to the department of Cardiology in our hospital in the past 3 years.The patients were diagnosed after four consultations and collected medical history and collected physical and chemical information during their hospitalization.We recorded their examinations,interventional data,and main adverse cardiovascular events(MACE)during hospitalization,and then a retrospective study was fellowed to summarize the distribution of AMI patients’syndromes,pathological conditions,and short-term prognosis,and focusing on heat poision syndrome The risk factors of heat poision syndrome were analyzed by univariate and multivariate logistic regression analysis to provide basic and clinical data for better guidance of clinical practice.Methods:From January 2015 to January 2018,who were hospitalized in the Department of Cardiology in our hospital diagnosed as AMI and met the inclusion criteria,we used the system to collect detailed information of the patients by logging in the Jiahe case system,telephone contact and clinic follow-up and then to fullfill AMI patient questionnaire and fellowed by data entry,lastly univariate,multivariate logistic regression analysis was conducted.Results:1.In this study,a total of 327 cases were collected,including the youngest 22 years old,the largest 93 years old,the average age of 59.76±12.99 years;266 cases of men,accounting for 81.3%,61 cases of women,accounting for 18.7%;There were 137 heat poision syndrome cases,accounting for 41.9%,and there were 190 non-heat poision syndrome cases,accounting for 58.1%.In the acute stage of AMI,there are 252 cases(77.1%)with TCM excess syndromes,and 74 cases(22.7%)with integrated excess and deficiency syndrome.Among them,the most common cases are thermal toxic and blood stasis syndromes,and there are 136 cases,accounting for 41.6%,followed by reciprocal certificate,which was 109 cases,accounting for 33.3%.The most common cases in integrated excess and deficiency syndrome was qi deficiency and blood stasis syndromes,which was 68 cases,accounting for 20.8%.2.Differences in pump failure,malignant arrhythmia,mechanical complications,hypotension,pulmonary infections,and hospital deaths between heat poision group and non-heat poision group,there was a statistically significant difference in the incidence of functional failure,hypotension,and pulmonary infection between heat poision group and non-heat poision group(P<0.05).The incidence of pump failure(37.4%)、the prevalence of hypotension(31.6%)and pulmonary infection(24.7%)were more higher in non-heat poision group than that in.The incidence of pulmonary infection(24.7%)was significantly higher than that in heat poision group,which the incidence of the fommer three accounting for 18.2%、13.9%、13.9%respectively.There was no significant difference in malignant arrhythmia,mechanical complications and death rate during hospitalization between heat poision group and non-heat poision group(P>0.05).3.Among the 327 subjects,301 cases were STEMI,accounting for 92.0%,and 26 cases were NSTEMI,accounting for 8.0%;LM,LAD,LCX,RCA,and MACE were 16 cases(4.9%)and 274 cases(83.8).%),174 cases(53.2%),216 cases(66.1%)and 45 cases(13.8%);110 cases of single-vessel disease,accounting for 33.6%,115 cases of double-vessel disease,accounting for 35.2%,three-vessel disease 101 cases,accounting for 30.9%.There was no statistical difference in myocardial infarction type and coronary vascular disease between heat poision group and non-heat poision group(P>0.05).4.There was no statistical difference in the cardiac ultrasound parameters between heat poision group and non-heat poision group(P>0.05).5.Univariate analysis:In sex,age,smoking,drinking,and family history a series of risk factors,there was a statistically significant difference only in sex,age,and family history between heat poision group and non-heat poision group(P<0.05),and the proportion of women in the non-heat poision group(25.3%)was significantly higher than that in the heat poision group(9.5%);the average age of the non-heat poision group was 63.26±13.24 years,significantly older than that of heat poision group 54.91±10.85 years,the proportion of family history in non-heat poision group(7.9%)was significantly lower than that of the heat poision group(15.3%);there was no statistical difference in smoking,drinking,hypertension,diabetes,and arrhythmia rates between the two groups(P>0.05).There was a statistically significant difference between hs-CRP and Lp-PLA2 between heat poision group and non-heat poision group(P<0.05),and the hs-CRP and Lp-PLA2 in hot-heat poision group was higher than that in non-heat poision group,while there was no statistically different in other indicators(P>0.05).6.Multiple logistic regression analysis results:From the above univariate analysis,we found that there was a statistically significant difference in sex,age,family history,hs-CRP,and Lp-PLA2 between the heat poision group and non-heat poision group.Therefore,a non-conditional,two-class,logistic regression analysis was performed using the heat poision group and non-heat poision group as the dependent variables,gender,age,family history,hs-CRP,and Lp-PLA2 as independent variables.After examination,χ2=34.653,P<0.05,suggesting that the regression model was statistically significant,and only age was found in gender,age,family history,hs-CRP,and Lp-PLA2 as independent heat poision syndrome factor.The OR was 0.947,less than 1,indicating that the age was a protective factor of heat poision syndrome,namely the older,the lower incidence of hot toxic syndromes.Conclusion:In the acute stage of AMI,the syndromes of TCM are mostly excess syndrome and integrated excess and deficiency syndrome.The distribution of syndromes is hot blood and stasis syndrome,reciprocal certificate,and qi deficiency and blood stasis syndrome.The heat poison syndrome is related to age,family history,hs-CRP and Lp-PLA2.After further multiple logistic regression analysis,we found that age is an independent factor of heat poison syndrome.The older,the lower the incidence of heat poison syndrome,suggesting that we should pay special attention to the young so as to identify the existence of heat-poison syndrome.Detoxification must be emphasized in the treatment.However,for the older,especially non-heat poison syndrome patients,they should be alert to the occurrence of pump failure,hypotension,and pulmonary infections.Once these occasion occur,we must do whatever we can as early as possible in order to improve prognosis. |
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