Analysis Of Metastatic Rule Of Adenoid Cystic Carcinoma Of Salivary Gland And Study On The Mechanism Of Curcumin Inhibiting Invasion And Metastasis Of Adenoid Cystic Carcinoma

Part 1:Analysis of Metastatic Rule of Adenoid Cystic Carcinoma of Salivary GlandObjective:To investigate the rules of cervical lymph node metastasis and distant metastasis of salivary adenoid cystic carcinoma(SACC)and their influencing factors,so as to provide a basis for its reasonable treatment.Methods:Clinical and follow-up data of 144 cases of SACC were retrospectively analyzed.All patients underwent radical surgery.Enumeration data was calculated using ?2 test or Fisher's Exact Test,Kaplan-Meier method was used to calculate survival rate,log-rank test was used for comparison between groups,and Cox proportional hazard model was used for multivariate analysis.Results:The overall 5-year and 10-year survival rates of the whole group were 77.4%and 51.9%,respectively;the COX model showed a higher T stage(OR 8.027,95%CI 2.479?25.992;P=0.001)and lymph node metastasis(OR 4.260,95%CI 1.072?10.664;P=0.002),positive margin or near margin(OR 1.886,95%CI 1.345?2.645;P<0.001)were independent risk factors for shorter survival time of SACC,and radiotherapy was an independent protective factor(OR 0.427,95%CI 0.214?0.851;P<0.016).A total of 23 patients(16%)had lymph node metastasis.Multivariate analysis showed that they originated from the oropharyngeal site(OR=10.709,95%CI:1.169?98.079,P=0.036)and higher T stage(OR=7.676,95%CI:1.513?38.952),P=0.014)were the independent risk factor for SACC lymph node metastasis.A total of 44 cases(30.6%)had distant metastases during the follow-up,of which 38 cases(86.4%)had lung metastases.COX multivariate analysis showed a higher T stage(OR=2.814,95%CI:1.051?7.677,P=0.04),Lymph node metastasis(OR=5.915,95%CI:2.451?14.274,P<0.001)and positive or close margin(OR=2.128,95%CI:1.431?3.165,P<0.001)were independent of SACC's shorter metastasis-free survival time Risk factors.Conclusion:Although the risk of cervical lymph node metastasis in SACC was not high,lymph node metastasiswas an independent prognostic factor of SACC.A higher T stage and tumor originating from oropharynx were risk factors for lymph node metastasis.It is recommended prophylactic cervical lymph node dissection to these patients.SACC is prone to distant metastasis,especially lung metastasis and higher T stage,lymph node metastasis,and positive/proximal margin are important risk factors for distant metastasis of SACC.SACC with distant metastasis lacks effective treatment and has a poor prognosis,and its reasonable treatment needs further study.Part2:Preliminary study on curcumin inhibiting the invasion and metastasis of adenoid cystic carcinoma through JAK-STAT3 signaling pathwayObjective:To explore the effect of curcumin on proliferation,invasion and metastasis of adenoid cystic carcinoma cells and its related mechanism.Methods:The CCK8 proliferation test was used to detect the effect of curcumin on the proliferation of adenoid cystic cancer cells.The Transwell invasion test and Scratch test were used to detect the effect of curcumin on the invasion and migration of adenoid cystic cancer cells.The effect of curcumin on apoptosis of adenoid cystic cancer cells was detected by flow cytometry.The expression of key molecular regulated by curcumin in STAT3 signaling pathway was detected by lentiviral interference technology,real-time quantitative PCR,Western blot and other technologies.Results:Cell experiments confirmed that curcumin can significantly inhibit the proliferation,invasion and metastatic ability of adenoid cystic carcinoma SACC-LM cells,and promote the apoptosis of SACC-LM cells(P<0.05).WB detection found that the expression levels of STAT3 gene and its downstream related genes of SACC-LM cells were significantly down-regulated when treated by curcumin(P<0.05).After blocking the STAT3 signaling pathway with lentivirus interference,curcumin's ability to inhibit SACC-LM cell proliferation,invasion and metastasis and promote apoptosis was greatly reduced,and there was no significant difference from the control group(P>0.05).Conclusion:Curcumin may inhibit the proliferation,invasion and metastasis of adenoid cystadenocarcinoma cells and promote its apoptosis through the STAT3 signaling pathway.This finding may provide new directions and ideas for the treatment of adenoid cystic carcinoma.