The Study On The Role And Mechanism Of LncRNA SNHG25 In The Proliferation,Invasion And Metastasis Of Epithelial Ovarian Cancer

Background: Ovarian cancer is the second most common gynecological malignancy among women.It is currently considered that the etiology of ovarian cancer involves genetic and epigenetic changes,but its underlying pathogenesis aren't fully elucidated.Although lncRNA cannot encode proteins,they regulates protein synthesis,influences cell proliferation,differentiation and survival.LncRNA may act as an oncogene or tumor suppressor gene in cancer,and regulate cancer-related signal pathways.Recent studies have found that the lncRNA SNHG family plays a vital role in tumors.However,there are no reports on the relationship between lncRNA SNHG25 and malignant tumors at home and abroad.According to the TCGA database,lncRNA SNHG25 was differentially expressed in ovarian cancer and normal ovarian tissue.EOC is the main tissue type of ovarian cancer,and we designed this study to explore the relationship between lncRNA SNHG25 and EOC.Objective: To investigate the role of lncRNA SNHG25 in the process of proliferation,invasion and metastasis of EOC and its molecular mechanism,and try to provide theoretical and experimental basis for the early diagnosis and as a new therapeutic target in EOC.Methods: 1.Using q RT-PCR detected the expression of lncRNA SNHG25 in EOC and normal ovarian tissue,and the expression of lncRNA SNHG25 in ovarian cancer cell lines SKOV3,A2780,Hey,OVCAR3 and normal ovarian cell lines IOSE80.2.Using lentiviral vector plasmid transfected ovarian cancer cell lines A2780 and OVCAR3 to knock down the expression of lncRNA SNHG25 effectively.They were divided into knockdown group and negative control group.And the ability of proliferation,apoptosis,migration and invasion of ovarian cancer cells in each group was detected.3.The m RNA differences between the knockdown group and the negative control group were analyzed by RNA-seq technology and bioinformatics,and the downstream target genes of lncRNA SNHG25 were verified via q RT-PCR and western blot.4.Subcutaneous xenografts in nude mice were to further verify the relationship between lncRNA SNHG25 and ovarian cancer cell proliferation by measuring tumor size and immunohistochemical methods.Results: 1.The expression of lncRNA SNHG25 in EOC tissue was significantly higher than that in normal ovarian tissue,and its high expression trend in EOC tissue was closely related to the FIGO staging and histological grading of ovarian cancer.2.The expression of LncRNA SNHG25 in ovarian cancer cell lines SKOV3,A2780,HEY,OVCAR3 was higher than that of normal ovarian cell line IOSE80,especially in A2780 and OVCAR3 cell lines.3.Knockdown of lncRNA SNHG25 could inhibit the proliferation,migration and invasion of A2780 and OVCAR3 ovarian cancer cells,and promote the apoptosis of ovarian cancer cells.4.Knockdown of lncRNA SNHG25 could down-regulate the expression of COMP and also inhibit tumor growth in nude mice.Conclusions: 1.LncRNA SNHG25 is highly expressed in EOC tissues.And the later FIGO stage and higher histological grade of EOC show a tendency of high expression.2.LncRNA SNHG25 is highly expressed in ovarian cancer cells.SNHG25 can promote the proliferation,invasion and metastasis of ovarian cancer cells,and inhibit the apoptosis of ovarian cancer cells.3.LncRNA SNHG25 can promote the proliferation activity of ovarian cancer cells by up-regulating the expression of COMP via RNA-seq transcriptome sequencing technology and bioinformatics analysis,which is expected to become a new target for the diagnosis and treatment of epithelial ovarian cancer.