Identification Of TCP Transcription Factors In Moso Bamboo And Function Analysis Of PeTCP10

Moso bamboo(Phyllostachys edulis)has higher ecological,cultural,and economic values.However,it often encounters various adverse environmental stresses,such as drought and salinity,during growth and development,which seriously restricts the development of bamboo industry.Thus,the urgent problem is how to improve stress resistance of moso bamboo.Molecular breeding is a fast and efficient breeding method,which can greatly shorten the breeding cycle and improve the breeding efficiency.TCPs,the plant specific transcription factors,play key roles in plant growth regulation and stress respose.However,the biological function of TCP in moso bamboo is still unknown.In this study,TCP transcription factors were identified from moso bamboo genome using bioinformatics method,and PeTCP10,which significantly induced by ABA and abiotic stress was selected.The biological functions of PeTCP10 was studied using over-expressing transgenic Arabidopsis and rice.The results of this study are as follows:1.Sixteen TCP members were identified from moso bamboo,they were divedied in two groups:10 members in Class Ⅰ and 6 members in Class Ⅱ;Expression pattern showed that they could express in multiple plant tissues and different growth stages.Meanwhile,their expression levels were significantly induced in ABA,Me-JA and SA condition;2.PeTCP10,being significantly induced by drought and salt,was identified using bioinformatics mothed;3.Molecular characterization analysis showed that PeTCP10 was a nuclear localization protein with self activating activity in Y2H Gold yeast.Y1H assay showed that PeTCP10 could bind to GGACCCAC element.Additional,yeast two-hybrid experiment showed that PeTCP10 could interact with two TCP members,namely PeTCP2 and PeTCP13.4.Over expressing PeTCP10 increased the drought and ABA toleranc of transgenic Arabidopsis;Overexpression of PeTCP10 induced stomatal closure in leaves under exogenous ABA condition,indicating that PeTCP10 may enhance drought resistance of transgenic plants by regulating stomatal movement.5.PeTCP10 might regulate lateral root growth via Me-JA signaling pathway;6.RNA-seq results showed that PeTCP10 could improve the drought resistance of transgenic Arabidopsis thaliana by regulating the expression of stress response related genes;In addition,a downstream target gene BT2 of PeTCP10 in transgenic Arabidopsis thaliana was identified by yeast one-hybrid and EMSA experiments;7.Overexpression of PeTCP10 enhanced the tolerance of transgenic plants to salt stress via enhancing the scavenging ability of peroxide in transgenic Arabidopsis thaliana.8.Overexpression of PeTCP10 could improve the tolerance of transgenic rice to drought stress at vegetative and germination stage,meanwhile improved the tolerance salt stress at germination stage.Overexpression of PeTCP10 improved the drought and salt stress tolerance of transgenic plants,indicating that it might play an important regulatory role in the osmotic stress response of moso bamboo;This result was helpful to enrich the theory of the function to environmental stress response of TCP transcription factor and provided new biomaterials and theoretical basis for stress-resistant molecular breeding of moso bamboo.