MiR-22 Regulates FASN To Inhibit Malignant Progression Of Melanoma Research

PurposeTo explore the role of the tumor-promoting gene FASN in melanoma,and to verify that miR-22 regulates FASN in melanoma to inhibit the malignant progression of melanoma.MethodBioinformatics techniques were used to predict miRNAs melanoma might bind to them,and then possible miR-22s were selected for validation.The expression levels of miR-22 in melanoma were detected by 40 pairs of clinical samples,and the correlation between miR-22 and FASN expression was detected by Pearson correlation analysis;the binding relationship was verified by dual-luciferase reporter gene assay.To explore the role of miR-22 in melanoma,we transfected M21 and A375 cells with miR-22NC,miR-22 mimics and miR-22 mimics+FASN-OE(overexperssion),and detected cells in each group by qRT-PCR.The expression level of miR-22 was then tested by CCK-8,EDU proliferation assay,Transwell cell migration assay and Invasion cell invasion assay to verify the effect of miR-22 on the proliferation,migration and invasion capabilities of melanoma cells.qRT-PCR and Western blot were used to detect the mRNA and protein expression levels of FASN in melanoma,respectively.ResultIt was predicted by bioinformatics methods that miR-22 may bind to the tumor-promoting gene FASN.After further verification,it was found that the expression of miR-22 was reduced in melanoma tissues and cells,and the expression of FASN was up-regulated in melanoma tissues.At the same time,Pearson correlation Analysis showed that miR-22 was negatively correlated with the expression of FASN.Cytological experiments proved that FASN promotes the proliferation,migration and invasion of melanoma.Experiments with dual luciferase reporter genes found that miR-22 had direct targeting binding to the 3’-UTR of FASN.Compared with the miRNA-NC group,the expression of miR-22 in the cells of the miR-22-mimics group was significantly increased,while the FASN mRNA and protein expressions were significantly decreased.Compared with the miRNA-NC group,transfection of miR-22-mimics significantly inhibited the proliferation,migration and invasion of melanoma cells.In addition,compared with the transfected miR-22-mimics group,the miR-22-mimics+FASN-OE group reversed the ability of miR-22-mimics to inhibit the proliferation,migration and invasion of melanoma cells.ConclusionmiR-22 is lowly expressed in malignant melanoma,and overexpression of miR-22 is target FASN to inhibit the proliferation,migration and invasion of malignant melanoma cells,and miR-22 is atherapeutic target for malignant melanoma.