Spatiotemporal Expression Of D10Wsu52e In The Developing Organs Of Mouse Foregut Endoderm

D10Wsu52e is a recently discovered mouse gene which is conserved from C. elegans to mammalia. FAAP, the protein encoded by D10Wsu52e, participates in regulation of integrin-based focal adhesions. FAAP is a highly homologous protein to the human protein HSPC117, and is further shown to inhibit cell spreading through regulating vinculin-paxillin association in integrin-based focal adhesions. To explore the function of FAAP in mouse embryonic development, we assessed the spatiotemporal expression of D10Wsu52 in the developing mouse embryo through quantitative RT-PCR, in situ hybridization and immunohistochemistry methods. Our results show that, at E9.5 and E10.5, D10Wsu52e mRNA is mainly expressed in the brain, optic vesicles, otic vesicles, limb buds, somites and gut, and also in the pancreatic buds at E10.5. Subsequently, D10Wsu52e mRNA is expressed mainly in the epithelial progenitor cells at E12.5, then decreases in the endocrine precursors and ductal cells, whereas still maintains in the exocrine precursors until E15.5. At E17.5, D10Wsu52e mRNA is highly expressed in exocrine acinar cells, but weakly in ductal and endocrine cells. Furthermore, the expression patterns of paxillin and vinculin mRNAs are similar to D10Wsu52e from E12.5 to E15.5, but not in E17.5, which suggests that D10Wsu52e may be related to the acinar development, adhesion and migration of progenitors and endocrine precursors.At E12.5 and E15.5, D10Wsu52e gene is highly expressed in the epithelium of stomach and duodenum, whereas at E17.5, D10Wsu52e is expressed mainly in the epithelium of villiform cacumen of stomach, and the expression of D10Wsu52e decrease in duodenum, although it is still expressed in all the epithelium of villus and crypts. The spatiotemporal expression pattern of D10Wsu52e in mouse stomach and duodenum suggests that D10Wsu52e may play a role in buds formation, organ growth and epithelial differentiation of stomach and duodenum.