The Expression Analysis Of GRB10 During Mouse Embryonic Development

The imprinted Grb10 domain on mouse proximal chromosome 11, it transcripts into 5015bp length mRNA, and then expressed growth factor receptor bound protein 10. Grb10 forms a link between fetal growth and glucose-regulated metabolism in postnatal life and is a candidate for involvement in the process of fetal programming of adult metabolic health. In addition, Grb10 is a potent growth inhibitor. To address the gene's expression level in different tissues during gestation process, we carried out the gene expression analysis by the real-time PCR and in situ hybridization analysis in fetuses and placentas of mouse. The analysis result showed that Grb10 is expressed all through the development of embryo at a high level, and the highest level expression of this gene was observed in E13.5 whole embryo. Furthermore, we make a contrastive analysis in many different tissues and organs at E13.5, E15.5, E16.5, E18.5 and placentas from E9.5 to E18.5. The results show that placenta, brain, heart, liver, intestines, kidney, spine, tongue, tooth bud, muscle, and gland have different level expression of this gene. Stronger singles of Grb10 was observed in brain, digestive tract epidermis, spine, kidney, and muscle tissues. In addition, Grb10 is mainly expressed in the continuously thickened labyrinthine trophoblast layer in early phase development of placenta, while in the latter phase, signals focus in the spongiotrophoblast layer and the amniotic membrane region. These results suggest that Grb10 plays an important role during the development process, especially for the early embryo nervous system and muscle skeleton growth in the mouse and development of labyrinthine trophoblast layer in the placenta.