The Expression Of Extro Growth Hormone Releasing Factor Mediated By Protoplast In Muscle Of Rabbit And Mouse

Growth Hormone Releasing Factor is Polypeptide hormone releasing by animal hypothaiami. It can induction synthesis and excretion of growth hormone which rise growth hormone level of animal. We large-scale cultured JM-109 strain recombinant plasmid of GRF. Then the plasmid was extracted from them and determined by DNA calculator. The protoplast that contain growth hormone releasing factor injected into rabbit muscle and mouse muscle after it were treated by 1% Glutaral,pay it to electric stimulation in muscle of injection site and extract omni-RNA from injection site of rabbit muscle,expression of GRF were detected by reverse transcription and PCR;ratio of expression of GRF were detected by ELISA. Extract DNA form injection site of mouse muscle to research time of expression .The result of investigation showing:extro growth hormone releasing factor mediated by protoplast expressed in rabbit muscle and mouse muscle,products of expression were detected by PCR RT-PCR and ELISA. Electroporation will acceleration cellular confluence of protoplast and muscles,but the significant changeof the ratio of expression and the time of expression was not observed form exponent treat with low pressure and short impulse of 100V,50 millisecond. Extro growth hormone releasing factor mediated by protoplast could more than 28 days retention in rabbit and mouse muscle that is condition of long-time expression of GRF.