Studies On The Effect Of ROS On In Vitro Early Embryonic Development Block In Mouse

In this experiment, we discuses the reason of the development block of the mouse early embryos and offered the reliable theories for enhancing the developing efficiency of the early embryos of the mammals by investigating the effects of ROS and antioxidant-GSH on the development of 2-cell embryo of mouse in vitro. We studied the effect of H2O2 on 2-cells mouse embryo development block in vitro by adding exogenous oxidant-H2O2 in different developing periods. The 2-cells embryo was gained from the oviduct of mouse, which was superovulated, and was cultured in vitro. The results of this study show:(1) the blastula developing rate of the control group and the experiment group which the concentration of hydrogen dioxide is 1.2 X 10"5% and 1.3 X 10~5, respectively, is significantly higher than the experiment group which the concentration of hydrogen dioxide is 1.4 X 10'5% , 1.5 X 10'5 and exceed 1.7 X 10'5 (P < 0.05) .(2) the blastula developing rate of the experiment group which was washing with hydrogen dioxide which the concentration is 1.4 X 10"5% during 0~ 12 hours and 0~48 hours is significantly lower than others groups( P < 0.05 ).(3) the blastula developing rate of the experiment group which is added GSH is significantly higher than the none. And under the situation that these is exogenous hydrogen dioxide( 1.4X10"5% ), with the increase of the concentration of exogenous antioxidant, the blastula developing rate also increase significantly.The ROS (such as H2O2) can inhibit the development of embryos in vitro. Thisfunction is outstanding in the period of development block of embryos. This suggeststhat the ROS is one of the reasons that induced the development block' of the mammals' early embryo.