Gene Mapping And Functional Analysis Of The Osdp Mutant

The development of floral organs, which is the most notable character ofangiosperms in their reproductive stage, has drawn particular attention from biologistsand becomes one of the focuses in plant molecular biology.During the heading stage,the individual plants in the F1 and F2 progenies from the cross between Osdp andGuangLuAi were investigated. In the F1 progenies, all plants exhibited wild-typephenotype, suggesting that the mutant trait is recessive. In the F2 populations, thesegregation rate of wild-type and Osdp plants fit the ratio of 3:1. Therefore, thedepressed palea trait is controlled by one recessive gene, termed Oryza sativa L.depressed palea (OsDP).In favor of map-based cloning,OsDP was located in theregion of 77kb which is between the molecular InDel markers CH910 and CH911 onthe BAC clone AP005093 of the chromosome 9. sequencing analysis of the resultantDNA fragment revealed that the deletion region is limited in the region of the 42Kbwhich is between the terminator of the Gene 1 and the terminator of the Gene7.Informatics analysis show that Recruitment of the members of the TCP family toplay new roles during plant development may underlie some key morphologicalchanges during angiosperm evolution.The mutant of the OsDP gene which should be anA function gene for rice floral organ identity leads to depressed palea.