| Atractomorpha sinensis Bolivar, species of Pyrgomorphidae (Orthoptera: Acridoidea), distributes all over the country, furthermore, it is one of the dominant species in some regions. As the polyphagy insects, they damage some plants including food crop, cash crop, vegetables and so on, which belong to the agriculture pest. Other respect, it is one of the traditional edible locusts in our country, which possess a high nutritive value and have deploiting and utilizing vales in some degree. Using the materials of Atractomorpha sinensis and methods of cytology, morphology anatomy and lectin histochemistry, this paper give a systematic study about the process of gametogenesis, the distribution of glycoconjugates during gametogenesis and the microstructure of spermatheca. The investigation may provide useful evidence for future researches in this field.The study, for the first time, has taken a microstructure's observation on the process of spermatogenesis of Atractomorpha sinensis in Orthoptera, by using the Hematoxylin-Eosin dyeing method. The results show that the process of spermatogenesis in Atractomorpha sinensis may be divided into 5 stages including the spermatophores proliferation stage, the primary spermatocyte stage, the secondary spermatocyte stage, the spermateliosis stage and the spermiotiliosis stage. Great changes in the size, morphology and chromosome of spermatogenic cells have been covered by those these 5 stages. Furthermore, chromatin condensation has been taken place in the late stage of spermatogenensis, which may correspond to the activities function of the sperms. By using the same method to investigate the microstructure of oogenesis , the result can be obtained that the process of oogenesis in Atractomorpha sinensis can be divided into 3 phases including 8 stages. The 3 phases is composed of oocyte differentiation phase, the oocyte growth phase and the phase of yolk formation. During the whole process, distinct changes have been found in the morphology of oocyte and follicular cells. They both contribute to the formation of yolk protein, from which we can concluded that the formation of yolk protein is an autosynthetic and heterosynthetic process. Additional, oocyte apoptosis occurred in the phase 2 of oogenesis in Atractomorpha sinensis, which may be an adaption to the disadvantageous environment.For further understanding of distribution and changes of glycoconjugates during oogenesis as well as spermatogenesis, 5 lectin-biding receptors have been detected in germ cells by using biotin-labled lectins including RCA I, PNA, WGA, UEA and SBA. The results showed that:During the process of oogenesis in Atractomorpha sinensis, there is no distribution of UEA receptor, while all the receptors of RCA I, PNA, WGA and SBA have taken great roles in oogenesis. The four receptors present as grade-distribution, and possess different distribution patterns respectively. Concrete distribution patterns appear as follows: Phase 1 with only RCA I receptor distribution; phase 2 with receptors of WGA, SBA, PNA present being the greatest distribution; phase 3 with the decreasing distribution of glycoconjugates. PNA receptor present only at stage 2 of phase 2 and stage 5 of phase 3. In the matured egg, receptors of RCA I, PNA, WGA distributed again in the vitelline membrane, which maybe take great roles in the recognition of egg and sperm. During oogenesis, there would be appearance of some glycoconjugates and disappearance of others, which may greatly relate to the growth of oocyte and the preparation for yolk formation .Furthermore, the 4 receptors also distribute in the follicular cells, which would make difference in the differentiation of their own and the maturation of oocyte.The result of glycoconjugates distribution in spermatogenesis of Atractomorpha sinensis shows that: there is no distribution of PNA receptor, and the other 4 receptors, with different distribution patterns, join the process of spermatogenesis. UEA receptor is found only in the spermateliosis and spermiotiliosis stage; the other 3 reacted receptors, synthesized by spermatophores, distribute on the membrane of spermatogenetic cells. Among these spermatogenetic cells, the secondary spermatocyte possess the greatest quantities of glycoconjugates which distribute discontinuously. At the stage of spermateliosis and spermiotiliosis, the glycoconjugates have experienced great modification and change: the disappearance of SBA receptor, the appearance of UEA receptor and the decreasing of the other 2 receptors. The modification and changes of the glycoconjugates would be responsible for the growth and maturation of the spermatogenetic cells.The morphology and microstructure of the spermatheca in Atractomorpha sinensis have been investigated by HE method in this paper. The result indicates that the spermatheca could be subdivided into a highly coiled spermathcal tube, a vestibule of...
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