Study On Driving Effect Of Plant Carbonic Anhydrase On Dissolution Of The Limestone

Carbonic anhydrase(EC 4.2.1.1),a ubiquitous metal enzyme that can catalyze thehydration reaction of CO2 , is one of enzymes whose catalytic velocity are confirmed to bethe fastest . In order to evaluate the function of carbonic anhydrase of in the karst area, thestability of carbonic anhydrase of typical plant in the karst area, effects of mainenvironmental factors on extracellular carbonic anhydrase of Chlamydomonas reinhardtii,drive function of extracellular carbonic anhydrase of C. reinhardtii on dissolution oflimestone and effects of carbonic anhydrase of plant and extracellular carbonic anhydraseof C. reinhardtii on leaching and migration of Ca and Mg were investigated. And the resultswere as follows . 1.Carbonic anhydrase of Vitex negundo in the karst area showed good stability to heatand good adaptability to intimidation of metal with high concentration such as Mg2+,Ca2+,Zn2+,Mn2+,Cu2+ and Co2+. 2.C. reinhardtii was confirmed to show adaptability to environmental factors such aslong time sunlight, high average temperature and high pH value and inorganic ion with highconcentration in karst area and its extracellular carbonic anhydrase have a high activity. 3.Driving effect of carbonic anhydrase of C. reinhardtii in calcareous soil systems wascarried out and the results showed that its function was evident. And relative analysisrevealed that there had a positive correlation between the leachates of Ca2+ and Mg2+ inKarst and extracellular carbonic anhydrase activity and biomass. 4.Correlations between C. reinhardtii and extracellular carbonic anhydrase activity andleaching and migration of Ca2+ and Mg2+ were investigated by simulating calcareous soilsystems with soil column. And the results showed that there was a positive correlationbetween C. reinhardtii and extracellular carbonic anhydrase activity and leaching andmigration of Ca2+ and Mg2+. Extracellular carbonic anhydrase of C. reinhardtii played an IIimportant role in the leaching of Ca2+and Mg2+. The total content of ions in the totalleachates collected from soil columns leached by inactive enzyme liquor was less than thatby enzyme liquor distinctly.