| In order to strengthen accumulation of oil in algae physically, the growth ofchlorella and the effect to magnetobiology of oil accumulation were exploredpreliminary by disposing chlorella with the static magnetic field in a short time. At thesame time, in order to study the response mechanism of microalgae for magnetic field,The activation of cell, the influence of cell cycle, the expression of cryptochrome gene(CPH1) and pCA under the blue light of magnetic field treatment were researched withmodel organisms Chlamydomonas reinhardtii as materials. It was explored whether thecrypotochrome which has been proved to be blue light reliant magnetic sensors in birdsand fruit body could affect the basic life activities of the C. reinhardtii. It tried toprovide important massages to molecular mechanism of biological response inmagnetic field. The mean contents as follow:The C.pyrenoidosa No.1,C.pyrenoidosa No.2,C.vulgaris NO.1and C.vulgarisNO.2were treated with the alternating magnetic field and static magnetic field for ashort time. The results showed that three algae strains of C.pyrenoidosa No.1、C.pyrenoidosa No.2and C.vulgaris NO.2had no significant difference with thetreatment of magnetic field on the accumulation of the biolipids. The oil accumulationof C.vulgaris NO.1was promoted by using150mT of the constant field and wasstrengthened by four magnetic flux density gradient of the alternating magnetic field. Itprovided reference data on strength of magnetic field for the microalgae’s enrichmentcultivation in large scales.A convenient, quick and accurate MTS method had been established to detect thealga cell activity. The activation of algae cell was characterized rapidly and accuratelyby MTS at37℃for5min. At the same time,the results showed that the enzymes ofalgal photosynthesis has no effect on the MTS determination of algal cell activity. Itsettled the foundation to explore mechanism of biological response quickly.The C. reinhardtii after cell cycle synchronization was treated24hours in differentgradient of tsteady-state magnetic field in blue light, The activation of cell, theinfluence of cell cycle and the expression of cryptochrome gene (CPH1) wereinvestigated. The results showed that in condition of100mT,200mT and300mT staticmagnetic field with the280lux blue light, The cell activity and cell cycle of C.reinhardtii displayed non-significant under magnetic field, but the expression of CPH1showed enhancement at300mT of magnetic field for12hours, and along with the processing time increased, CPH1expression quantity did not show the differencecompared with control.We also detected the activity of the extracellular carbonic anhydrase (pCA) andexamined the CA enzyme gene (CAH1) expression levels with magnetic fieldtreatment. The results showed that under100mT,200mT,300mT steady magnetic fieldconditions with the irradiation of the280lux blue light, CA activity of C. reinhardtiidid not show a significant response. It also found that the expression of CAH1wasinhibited obvious at300mT of magnetic field for8hours. |
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