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Research On Nuclear Basic Protein During The Process Of Sperm Formation In Eriocheir Sinensis

Posted on:2007-11-01Degree:MasterType:Thesis
Country:ChinaCandidate:D D MaFull Text:PDF
GTID:2120360182485715Subject:Biochemistry and Molecular Biology
Abstract/Summary:PDF Full Text Request
Nuclear basic proteins that are responsible for chromatin condensation and conservative for hereditary information of DNA are very vital in the process. Round spermatid will be elongated, and its chromatin undergoes substantial compaction during spermiogenesis.The researches of nuclear basic protein begin with the fish protamine. At present, a depth study is developing in many aspects. These findings displayed all sorts of amino acid composition, quantity and proportion, which is important for species evolution. Especially, Decapoda spermatozoa are different from that of most animals, with nucleus being non-condensing. The phenomenon is widespread in Decapods, and attracts many scientists to research. About the research of non-condensing sperm nucleus, they focused on light microscope. With the development of electron microscopy, the further study was started.This paper have studied freshwater organism - E. sinensis , which is economic crab in China, with the common characterized of Decapods. It is unknown about category and character of nuclear basic proteins displacement in spermiogenesis. We will explore these questions of above by followed:1. We have studied histological of testis in E. sinensis and it is the results that the paired testes contain vast number of coiling convoluted seminiferous tubule which show different stages of spermatogenesis. The germinative area lies on one side of the liners of the tubules and produces spermatogenic cells in batches to form various spermatogenic cell belts. These germ cells develop and move to the tubule antrum. Finally, the mature spermatoblast or sperm enter into the vas deferens. At the same time, the studies demonstrated that the formation of maturation of the testis is similar to that of the germ cell in the same convoluted somniferous tubules. The crest of the testis mature first, from this part, the testis develop and mature from its outfield to endothecia, in the same way, the mature spermatoblast or sperm enter into the vas deferens. Apart from this, it gives a description to the histology structure of the testis of E. sinensis, and divides the testicular development cycle into 5 periods: the...
Keywords/Search Tags:Eriocheir sinensis, testis, spermatozoa, nuclear basic protein, SDS-PAGE, AU-PAGE, biochemical characteristic
PDF Full Text Request
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