The Refining Of Egg White Lysozyme

In this experiment, ultrafiltration and anion exchangeresin were used in the process of purifying the lysozymefrom egg white, including purification,identification anddetermination of enzyme activity.First, by using the elution buffer PBS(Na2HPO4 _NaH2PO4,pH8.5, I0.20mol/kg), the solution was homogenization. A withthe PES membrane(MW=30000Da), 0.35MPa of filtrationpressure, the buffer PBS was used to elute.The part of filtrated was performed by DEAC-celluloseanion exchange resin, under the condition of Trisbuffer(original pH10.0, I=0.1).Then the eluted part was collected, ultrafiltered againand desalinized with the pressure of 0.35MPa.The ultrafiltrate was dried by refrigeration. And thepurity was up to99.5%, enzyme activity was 15500U/mg, thefinal yield is 60%.