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Research On DNA Extracting Technology From Mammalian Faeces

Posted on:2007-05-31Degree:MasterType:Thesis
Country:ChinaCandidate:J Y ZhaoFull Text:PDF
GTID:2120360185484918Subject:Biochemistry and Molecular Biology
Abstract/Summary:PDF Full Text Request
Extracting DNA from faecal sample is a newly developed technology recent years. As an ideal noninvasive sampling way, it alleviates sampling problems which existed longtermly in conservative genetics and molecular ecology studies. The core of this technology is whether the high quality faecal DNA can be extracted from faecal samples. Although several methods to extract DNA from faeces had been developed home and abroad, it confirmed the possibility of faecal DNA analysis technology in a certain extent. Some questions below hadn't been solved yet. â‘ The currency of most methods is not good. In different experiments the researchers always got different amplify success rate between different animal species and individuals. This question hampered the development and spread of molecular scatology badly. At the same time the price of the kit for extracting faecal DNA is expensive. So it make sense to develop a new method which has good currency and cheap cost. The new method will accelerate the spread of molecular scatology. â‘¡Most researches addressed fresh samples (the faeces preserved for less than 24 months) and had not researches on DNA extracting from old faeces yet. Extracting DNA from old faeces is important for making full use of genetic information of died animals or migratory animals.
Keywords/Search Tags:Faecal DNA, current method, old faecal, faecal preservation, PCR amplification
PDF Full Text Request
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