| Tetrodotoxin (TTX) , also known as a potent marine neurotoxin in puffer fish , occurs in a variety of animal . Since the toxin can occur in marine commercial animals , they represent serious public health problems. As a result , continuous monitoring for the toxin in fish and seafood is required .The sample preparation is pivotal to determine TTX by HPLC, included the sample extraction and purification involving the recovery and impurity removing. Many sample preparations were parallel with different researchers'report . First , the tissues were extracted with acetic acid , then applied to columns of activated charcoal to remove impurities. The adsorption is very strong , so the TTX is hard to be eluted , resulting in low recovery. Then the extraction was ultrafiltrated though membrane( cut-off limit 1000MW) or applied to a Bio– Gel P-2 column to remove macromolecules. At last , the liquid was applied to cation exchange resin two or three times to get rid of impurity . The chromatogram is good , but the recovery is low . It is hard to determine the content of TTX. At this experiment , we study the purification effect of the D152 Macroreticular cation exchange resin and Cartridge of SPE C18, the results show that D152 washed by 5% acetic acid can get rid of 86.4% the overall sugar, and 62.0%of amino acid. The recovery of TTX is 97.5%. And the recovery of passing through the cartridges by acid-washing is 95.3%, RSD is 2.16%. By HPLC method, the chromatogram show that the peak of TTX apart from the matrix, and the recovery is high.At this experiment, a RP-HPLC method which associates with phosphate buffer...
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