| Somatic embryogenesis of Achnatherum splendens (Trin.) nevski in vitro was studied in this experiment. Somatic embryos were obtained by using solid and liquid suspension culture, with leaf sheath and hypocotyls as explants. A system of somatic embryo was established. Mechanisms of somatic embryogenesis of A. splendens (Trin.) Nevski were discussed by three aspects: histological observation, isozyme spectra and specific proteins. Furthermore, a system of regeneration in vitro by callus was established for A. splendens (Trin.) nevski.1. Embryonic callus (EC) was induced in the medium of B5+1.0 mg·L-1 2, 4-D+1.0 mg·L-1 NAA. A great deal of somatic embryos were induced in the medium of B5+ 0.2 mg·L-1 6-BA + 0.2 mg·L-1 NAA + 0.5 mg·L-1 ABA or B5 + 0.2 mg·L-1 6-BA + 0.5 mg·L-1 NAA + 1.0 mg·L-1 ABA with EC.The histological observation showed that somatic embryo of A. splendens occurred from the inside of EC.It's inner-origin. Somatic embryo of A. splendens contained a large amount of starch grains and proteins.The isozyme research showed that amylase, POD and ATPase changed during the course of somatic embryogenesis. Some specific spectrum bands were found during the developmental stages. It could be the biochemical markers of somatic embryogenesis.The protein analyses by 2-dementional electrophoresis showed specific proteins in different periods. The specific proteins of EC were 3- A (pH5.1, MW33kD); 3-B (pH4.9, MW28kD) .They were signs for somatic embryogenesis. The specific proteins of maturation embryo were 4-A (pH4.0, MW36kD); 4-B (pH4.0, MW34kD) .They showed that somatic embryos were completely developed.2. The experiment for organogenesis of callus showed that the medium containing B5+0.5mg·L-1 6-BA +0.2mg·L-1NAA was suitable for shoot induction. As for root induction, the medium of 1/4B5+1.0mg·L-1NAA+ 0.2mg·L-1IBA +1.0g·L-1active carbon (AC) was fit. This experiment got the high surviving rate of transplant.
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