The Preliminary Investigation On EPA Production Of Pinguiococcus Pyrenoidosus

Pinguiococcus pyrenoidosus CCMP 2078 is a lately named marine algae, which belong to Pinguiophyceae, Pinguiococcus genera. Analyzing the biochemical composition of this algae, results shows : the protein content is 37.54%, rude lipid content is 27.45%, PUFAs including 18: 2,18: 3,18: 4, AA (Arachidonic acid, 20:4) , EPA (Eicosapentaenoic acid, 20:5) , DTA(Docosatetraenoic acid, 22:4), DHA (Docosahexaenoic acid, 22:6). The characteristic of its lipid composition is the high amount of PUFAs especially EPA, but no (or trace amounts) those fatty acids (16:3, 16: 4, 18: 3 and 18: 4) have been found. The results indicate the commercial potential in EPA production from P. pyrenoidosus CCMP 2078 because of its higher value in nutrition aspect.In the present paper, effects of environmental and nutritional factors on the growth, fatty acid composition, EPA and PUFAs content of P. pyrenoidosus CCMP 2078 were investigated .Results indicated:(1)P. pyrenoidosus CCMP 2078 grew well under 20℃²8℃ condition. Higher temperature promoted algal growth. However, with temperature increase, EPA and PUFAs contents decreased.The highest value of EPA (20.83%) and PUFAs (39.16%) were found when temperature was at 20℃.(2) With light intensity transfer from 50 to 200μmol photons m^-2s^-1 the algal biomass and specific growth rate of the algae decreased. It grew fastest under light intensity of 50μmol photons m^-2s^-1 Growing under 50 to 150μmol photons m^-2s^-1, the EPA and PUFAs contents in the algae raised with the increase of light intensity. The highest EPA and PUFAs contents (4.92% and 13.25% respectively ) were reached at the light intensity of 150μmol photons m^-2s^-1. When the light intensity reached to 200μmol photons m^-2s^-1, the lowest PUFAs content (1.62%) was obtained and no EPA could be found.(3) The algae grew well in the salinity range from 25 to 40, while the growth was inhibited when the salinity decreased below 20. The highest EPA and PUFAs content (8.94% and 20.56% respectively) reached at the salinity of 20. Lower salinity (15, 20) was proper for EPA and PUFAs accumulation, while the salinity higher than 20 was not good for accumulation.(4)The biomass and specific growth rate increased with the pH increase from 5.0 to 9.0. The algae grew slowly at pH 5.0 and grew best at pH 8.0, while the pH 9.0 and 8.0 had no...