Molecular Classification And The Adaptative Mechanism Of Thermophilic Bacteria

Microorganism is the most abundant biology resource on the earth, and thermophile is one of the important members of it. These microorganism have developed an unique enzyme system and recovery mechanism to adapt to extreme environment, thus becoming an important biology resource for industrial use because of the heat-resistant of their cells and enzymes. The enzymes and the biological active materials may play an important role in gene engineering, protein engineering, fermentation engineering and exploiting of mineral resources. Moreover, thermophile has a special role in the biological systemic evolution. The study on their adaptation to the environment will be helpful for understanding the origin of life.We collected samples from a hot spring around Xiamen near the sea and the thermophilic bacteria were cultured at 75℃and 80℃, 60 strains were obtained in total. Traditionally, taxon of bacteria usually based on the phenotype that is time-consuming. So we applied three molecular methods to facilitate the process of classification including SDS-PAGE electrophoresis of whole bacterial cell protein, RAPD and amplification of 16S-23S rDNA spacer regions. By combining these three methods, we had isolated 7 strians that belong to different genus. These 7 different strains were further confirmed by sequencing the 16S rDNA and being uploaded to NCBI to compare with other bacteria that had been found before. These results provided a reliable basis for the further study on these thermophilic bacteria. Furthermore, combination of these 3 molecular methods could be used as a simple way to classify bacterial strains.Thermophilic bacteria grow at extreme high temperature. The adaptation to the fluctuation of temperature is important for them to survive and grow. Understanding the temperature adaptaton mechanism will also help to elucidate the relationship between thermophilic bacteria and mesophilic bacteria, and uncover the riddle of evolution. The thermophilic bacterium WH10 was examined to gain a better understanding of gene expression in response to low temperature. Random arbitrarily...