The Effect Of Decrease Of FGFR2 Expression By Conditional RNAi Knockdown Technology On The Development Of Mouse Cartilage

Gene knockout is an important method to investigate the role of gene.Geneknockout technology has already become a new molecular biology technology by theend of 1980's.As usually, Gene knockou is based on the, which use the designedgene fragment to take the place of target gene fragment to achieve the goal of geneknockout.With the development of gene knockout,new principle and technology isincreasingly used, such as,inducible gene knockout, conditional gene knockout,random insertion fragment for gene knockout, antisense nuclear acid technology.In recent years,some new technology is used in research on gene role,RNAiknockdown technology, as one of them,decreases gene expression and identifieseasily gene role by comparison of genetype. In this article, we investigate the impactof FGFR2 in mouse cartilage on the development of cartilage with conditional RNAiknockdown technology.Firstly, we identify the conditional FGFR2 knockdown FGFR2-RNAi mouseand Coll2a-Cre mouse expressing recombination protein Cre in cartilage.Then,wemake FGFR2-RNAi mouse mate with Coll2a-Cre mouse to produce offspringdouble transgenic mouse FGFR2RNAi; Coll2a-Cre identified by genetype, we havethe reaserch on the relationship of weight and time for female double transgenicmouse and female control and find that weight of female double transgenic mouse isless than female control in the same time after statistical analysis.For the maledouble transgenic mouse and male control, we have got the same result above.wefurther compare the width of female double transgenic and male double transgenic tocontrol respectively and find that the width of double transgenic mouse is less thancontrol in the same time after statistic analysis. We culture cartilage cell in vivo andanalyze RT-PCR of FGFR2 and find that the content of FGFR2mRNA in cartilage ofFGFR2RNAi; Coll2a-Cre mice is lower than that of controls.Therefore,all the resultsabove release that we can observe the development of cartilage with conditionalRNAi knockdown decreasing FGFR2 expression.