Screening Cellulose Degeneration Micro-Organisms Under Low Temperature

Cellulose is the most abundant and extensive source of organic materials on Earth. Due to the technicaland other reasons, this type of valuable resource has not been fully utilized, and some of them have even beendumped becoming environmental pollutants. In particular, in northern part of China, the low temperaturewith long winter slows down the degradation of cellulose. In order to improve the utilization efficiency ofcellulose, screening cellulose-degradation microorganisms under low temperature, including 22 strains ofbacteria and 21 strains of fungi, was conducted in the experiment. The results showed that 13 strains ofbacteria and 11 strains of fungi grew well under low temperature and were considered suitable forlow-temperature growth. Xinhua filter paper as the sole carbon source in the culture medium was used to dothe preliminary screening, and Congo Red medium was used to do the rescreening, which demonstrated that 6strains of bacteria and 6 strains of fungi had significant transparent circles with high cellulase activity.Through liquid culture and cellulase activity analysis, six strains had higher cellulase activity. It was identifiedthat B9 is classified as Aeromonas bacteria macrophages (Myxomycetes fiber Cytophage an alias); B21(Cellulomonas) as Aeromonas bacteria; F3 strain as small Alternaria spp (Syzygites Ehrenberg); F5 strain asRhizopus nigricans (Rhizopus Ehrenberg); F17 strain as the genus Trichoderma (Trichoderma); F21 as smallSclerotia.Antagonism test was conducted to show that those 6 strains selected were not against each other and B21promotes other five strains' growth. Liquid compound test showed that four conbinations (B9B21, F21F5,B9F3. F3F17) were good combinations, among them F3F17, F21F5, B9B21 were better portfolio.Culturing five conbinations on solid medium in the experiment showed that F3F17, F21F5 were idealcombinations, which had higher enzyme activity in cellulose degradation with degradation rate at 33.3%. Thedark brown color, surface pastivity and reduced toughness of the procucts showed good results were achieved.The six strains of the microorganisms grew well under low temperature with high cellulose degradationreates. The two conbinations F3F17,F21F5 could grow well under low temperature on the normalinexpansive solid growth medium and degrade cellulose in a relatively short period of time. Those results adda new power to the agricultural waste treatment as a resource utilization and environmental pollution reduction...