Culture And Microstructure And Ultrastructure Study On Fuligo Septica And Physarum Compressum

Two materials in this experiment are Fuligo septica(L.) Wiggers which belongs to Physarales, Physaraceae, Fuligo, and Physarum compressum Alb. & Schwein. which belongs to Physarales. Physaraceae, Physarum. Both plasmodia of these two species are phaneroplasmodia.Through the culture with sterile oat powder and water on the substrate of 2% water-agar, we can see that the plasmodia of Fuligo septica with big thick fan shape to swarm without few remifications were very strong, and they should be conveyed to new substrate over 10 days; but plamodia of Physarum comprssum with a not big thin fan shape and more remifications were very slim, they should be conveyed to new substrate over 15 days. The plasmidia's living condition of Physarum compressum is more strict than Fuligo septica especially in the fact of temperature.The results showed that Fuligo septica plasmodia are easy to form sclerotia, so that they were used to be the materials to study the microstructure and ultrastructure about transformation from plasmodia to sclerotium by drying and starving. The Physarum compressum plamodia are easy to form sporangia, so that they were used to be the materials to study the microstructure and ultrastructure about transformation from plasmodia to sporangium by hoisting the temperature. The major methods were to observe special dye to the organelles and isolated organelles and living organelles from microscope, and to observe organelles and negative stained isolated organelles from TEM.The results of microstructural and ultrastructural observations showed that plasmodia would be cleavaged to form spherules at the beginning of sclerotium formation. The spherules arranged closely and formed cuticles following the plasmodia assembled to plicate and dissipate the inner water.Through the observations to the Physarum compressum which was transforming to sporangia from plasmodia from TEM, we can found the inner structures were denser than the formers. The inner plasmodia were cleavaged to small spheres which were proto-spores following the proto-sporangia developed with plasmodia injected and the color changed from white to dark gradually. There was an electrondense layer between two closed spheres and two electron-lucent layers at the opposite sides of the electrondense layer. When the color of sporangia changed to deeply dark, we can see that the electrondense layer torn two layers, each layer became the outer sporoderms of the two neighbored spheres. After two joined spheres divided, we can see that the outer sporoderms had protruded wart and concave holloeness that adapted to each other, and these were the ornamentations of the matured spores. The results showed that some electrondense layers didn't divided into two layers but three layers, and the middle layer would became to capllitia after spores formed. This viewpoint is extremely different from traditional.This experiment also contains using fluorescence dye to observe from fluorescence microscope, and using Janus Green b to dye the living organelles to observe from microscope, both them had acquired better observation results. In addition, we also used special dye to dye the nuclei and isolated nuclei, we can see that the former manner was better than the last.This experiment created a new method to observe the shape of mitochondrion with tube-shaped crista clearly in the strong plasmodia of myxomycetes from TEM negative staining after twice low-speed centrifuges and twice high-speed centrifuges. Mitochondrion with tube-shaped crista is same to the protozoan, but different from fungi. This phenomenon can be one of the evidence to prove which the myxomycetes belong to.