Effect Of Environmental Factors On Endothelial Cell And Estabilshment Of EA.hy926 Phospholipid Profile

In order to elucidate the injury mechanism of endothelial cells, we employ the lipidomics strategy and signaling transduction together to study physical status changes after stimuli (TMP·HCl and Angâ…¡/ H₂O₂) of human umbilical vein endothelial cell line EA.hy926 and establish phospholipids profile of EA.hy926.The achievements in our study are as follows: (1) we detected enzyme activity changes and regulatory mechanisms of relative signals in the treatment of Angâ…¡and Tetramethylpyrazine Hydrochloride, the effective component of traditional Chinese medicine Ligusticum chuanxiong Hort; (2) we found there are at least 6 classes and 219 phospholipids molecular species in EA.hy926, and we establish a EA.hy926 phospholipid database basing on these results, and measure the relative contents of 219 phospholipids by NPLC/MS/MS; (3) we measured the changes of PLA₂ activity when cells injured by H₂O₂ and pretreated by TMP·HCl. Degradation of phosphatidylcholine by PLA₂ causes generation of lysoPC, an important second messenger of cell apoptosis. Results showed that the activation PLA₂ by H₂O₂ could be inhibited by TMP·HCl and also the lysoPC content exhibited the same trend. This demonstrated that PLA₂ seem to play a key role in oxidation injury of endothelial cells. These findings laid the foundation for further exploration of protective mechanisms of TCM in endothelial cell injury.