| The anterior subventricular zone(SVZa) is one of the regions that are abundant of neural stem cells in the brain. The precursors of the olfactory interneurons generated at the SVZa are characteristic of that, they don't differentiate into periglomerular cells and granule cells until they migrate tangentially, via the restricted route called the rostral migratory stream (RMS), to the olfactory bulb. Therefore, the SVZa provides a useful model to understand a process of general importance in the brain. Myosinâ…©, a vertebrate-specific member of myosin super-family, can be detected in most tissues and cells, such as kidney, liver, brain, and so on. Full-length Myosinâ…©can stimulate the formation or stabilization of filopodia. As an actin-based motor protein, Myosinâ…©plays a key role connecting cell membrane proteins and cytoskeleton. So it may play an important role in migration of the olfactory interneurons.In this research, firstly, make sure that there are more Myosinâ…©in RMS and SVZa through immunohistochemistry. Next, an explant of RMS culture system were set up, through mixing different proportions of collagen and matrigel complex to cultivate explants so that the best condition was optimized. To investigate whether Myosinâ…©has effects on neuronal migration, the SVZa neurons were transfected with the Myosinâ…©-siRNA, then re-aggregated and cultured and their migration distance and numbers are analysied 24h later. The migration distance and numbers of cultured re-aggregating SVZa neurons were changed by expression of Myosinâ…©- siRNA, whereas scramble siRNA had no effect. The results showed that the interference of Myosinâ…©made neuron lower the ability of migration. To sum up, Myosinâ…©plays an important role in the migration of neuron migration of SVZa.
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